Effects of prednisolone injection on the liver of the mouse inoculated with the adult worms of <i>Clonorchis sinensis</i> intraperitoneally

, Lee, Soon Hyung; , Song, Chul Yong; , Chi, Je Geun

doi:1978.16.2.69

Korean J Parasito > Volume 16(2):1978 > Article

Original Article


Korean J Parasitol. 1978 Dec;16(2):69-81. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1978.16.2.69
Copyright © 1978 by The Korean Society for Parasitology


Effects of prednisolone injection on the liver of the mouse inoculated with the adult worms of Clonorchis sinensis intraperitoneally
Soon-Hyung Lee,Chul-Yong Song and Je G. Chi
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.
Department of Pathology, College of Medicine, Seoul National University, Korea.



Abstract
In order to understand the effect of prednisolone injection on the histopathological changes of the mouse liver and the chronological changes of the worm structure of Clonorchis sinensis, when this fluke was inoculated to the mouse intraperitoneally. The recovery rate, survival rate, location and size of the inoculated worms as well as the histopathological changes of the liver were investigated for the comparison among the groups of mice, which were classified by number of worms and the duration of observation period. The result obtained were summarized as follows: 1. The recovery rate and survival rate of the worms decreased especially 28 days after the inoculation. 2. Most of worms (45.5%) were collected from the peritoneal cavity, and some of worms were found tightly adherent to the capsules of the liver, spleen, intestine and diaphragm. 3. The mean worm size after inoculation was constantly smaller than that before inoculation. 4. Remarkable atrophy in the reproductive organs of the worm, such as spermatheca, testes, vitelline gland and ovary was frequently observed at the 10th day of inoculation. 5. Histopathologically the liver failed to show any parasitic worm inside the intrahepatic biliary system. However, multiple well formed egg-containing granulomas were present along the liver capsule. These necrotic granulomas were occasionally found under the fibrotic liver capsule. Focal necrosis and focal phlebitis together with vascular dilatation were prominent features of the liver. 6. The worms recovered in the capsule of the liver were degenerated and necrotized. Usually, there were remarkable capsulitis and granuloma formation around the eggs.

Figures

Figs. 1-4
Fig. 1. A normal worm (C. sinensis) of control group.

Fig. 2. A recovered worm from the abdominal cavity of the mouse, 4 days after the intraperitoneal inoculation. The spermatheca began to atrophy.

Fig. 3. Atrophy of the spermatheca, testes, ovary, vitellaria and scantiness of egg in the uterus were observed in the worm, 10 days after the inoculation.

Fig. 4. Remarkable atrophy of reproductive organs was visible in this worm, 28 days after the inoculation.

Figs. 5-8
Fig. 5. Hepatic lobule showing irregularity of nuclear size. H&E×400.

Fig. 6. Dilated venous channel is seen in the center, the wall of which is infiltrated with various inflammatory cells. H&E×400.

Fig. 7. A granuloma possibly parasitic origin is observed under the liver capsule. H&E×100.

Fig. 8. Photomicrograph of a necrotic granuloma(presumed to be a dead worm) excavation th liver capsule. H&E×100.

Tables


	Table 1 Experimental design


	Table 2 Number of worms recovered


	Table 3 Number of worms survived


	Table 4 Worms recovered from various parts of the body by group


	Table 5 Worms recovered form various parts of the body by duration


	Table 6 Worm size after inoculation by group


	Table 7 Worm size recovered after inoculation by duration


	Table 8 Atrophic changes of reproductive organs of the worms found by group


	Table 9 Atrophic changes of reproductive organs of the worms found by duration


	Table 10 Histopathological changes of mouse liver

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