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Korean J Parasitol > Volume 21(1):1983 > Article

Original Article
Korean J Parasitol. 1983 Jun;21(1):41-48. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1983.21.1.41
Copyright © 1983 by The Korean Society for Parasitology
Aspartate and alanine aminotransferase in Fasciola hepatica
Sun Hyo Park,Nyon Soo Kwon,Hi Sung Lee and Chul Yong Song
Department of Biochemistry, College of Medicine, Chung-Ang University, Korea.
Department of Biology, College of Liberal Arts and Sciences, Chung-Ang University, Korea.
Abstract

The activity and distribution of aspartate aminotransferase (EC 2.6.1.1) and alanine aminotransferase (EC 2.6.1.2) in adult Fasciola hepatica have been studied. Fasciola hepatica was fractionated by differential centrifugation into nuclear, mitochondrial and cytosolic fractions. The activity of GOT and GPT was measured by the method of Reitman and Frankel. Isozyme patterns of those enzyme were also examined by DEAE-cellulose column chromatography. The results obtained were as follows;

1. The activity of aspartate and alanine aminotransferase was about 0.55 unit and 0.92 unit per 1 g of Fasciola hepatica, respectively.

2. The activity of those enzymes was relatively low compared with those in mammalian tissues.

3. The distribution of aspartate aminotransferase in the subcellular organelles showed that 71% of the activity was in cytosolic, 24% in mitochondrial and 5% was in nuclear fraction.

4. About 22% of the total alanine aminotransferase activity was found in the mitochondrial fraction, about 66% in the cytosolic fraction.

5. Aspartate aminotransferase from cytosolic fraction was separated into two types of isozymes, whereas alanine aminotransferase from cytosolic fraction gave only one active peak on DEAE-cellulose column chromatography.

Figures


Fig. 1
Chromatography of GOT on DEAE-cellulose. (•-•, protein; ○-○, enzyme activity).


Fig. 2
Chromatography of GPT on DEAE-cellulose. (•-•, protein; ○-○, enzyme activity).

Tables


Table 1
Distribution of aspartate aminotransferase (GOT) in Fasciola hepatica


Table 2
Distribution of GPT from Fasciola hepatica


Table 3
Isozyme patterns of aspartate aminotransferase from Fasciola hepatica


Table 4
Purification of aspartate aminotransferase from Fasciola hepatica


Table 5
Purification of alanine aminotransferase from Fasciola hepatica

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