Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Studies on intestinal trematodes in Korea XIV. Infection status of loaches with metacercariae of Echinostoma cinetorchis and their development in albino rats
| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasito Search

CLOSE

Korean J Parasito > Volume 22(2):1984 > Article

Original Article
Korean J Parasitol. 1984 Dec;22(2):181-189. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1984.22.2.181
Copyright © 1984 by The Korean Society for Parasitology
Studies on intestinal trematodes in Korea XIV. Infection status of loaches with metacercariae of Echinostoma cinetorchis and their development in albino rats
Byong-Seol Seo,Yang-Hee Park,Jong-Yil Chai,Sung-Jong Hong and Soon-Hyung Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110, Korea.
Abstract

The metacercariae of Echinostoma cinetorchis (Trematoda: Echinostomatidae) were found infected in the loaches (Misgurnus anguillicaudatus) purchased from two local markets in Korea. Identification of the metacercariae was done after obtaining adult worms from experimentally infected albino rats. Brief course of worm development in rats was also observed up to 28th post-infection day. The results are as follows: The metacercariae of E. cinetorchis were detected from 5 (10.9 percent) out of 46 loaches examined and the average metacercarial burden per infected loach was 34.4. They were always found from the posterior abdominal wall. The worm recovery rate from 8 rats was 24.6 percent in average. It was observed that the rate decreased according to age of infection; from 40.7 percent after 6 days to 15.0 percent after 28 days. The adult worms were morphologically characterized by the presence of head crown with dorsally uninterrupted collar spines, 37-38 in number, and the tendency of migration or disappearance of testes. So that they were identified as Echinostoma cinetorchis Ando et Ozaki, 1923. The development of worm was very rapid during 6-10 days after infection and became much slower thereafter. During the rapid worm growth the development of genital organs was more prominent than that of non-genital organs. From the results it is concluded that the loach is involved in the life cycle of E. cinetorchis in this country and may take a role for infection source to animals and man. It is also confirmed that migration or loss of testes is the distinct specific character of this echinostomatid fluke.

Figures


Figs. 1-4
Fig. 1. Metacercariae (arrow) of E. conetorchis in posterior abdominal wall of the loach, Misgurnus anguillicaudatus. ×40.

Fig. 2. Higher magnification of Fig. 1. ×100.

Fig. 3. Isolated metacercaria of E. conetorchis which shows well developed oral sucker (OS), ventral sucker (VS), refractile excretory granules (Ex) and end group spines (S). ×400.

Fig. 4. 6-day old juvenile worm of E. conetorchis showing ovary (O), Mehlis' gland (MG) and coiled uterus (U) which does not contain eggs.

** Scale unit in Fig. 4 is millimeter.



Figs. 5-8
Fig. 5. 10-day old adult worn. The rosette form uterus with numerous eggs and the witelline follicles have developed well.

Fig. 6. An adult worm recovered on 16th day after infection showing two testes (T), one of which noved to the postero-lateral portion of ventral sucker.

Fig. 7. 21-day old adult worn.

Fig. 8. 28-day old worn. Note that the number of intra-uterine eggs narkedly decreased and testes disappeared.

** Scale unit in Fig. 5-8 is millimeter.



Fig. 9
Growth curves of body length and width according to age of worms.


Fig. 10
Growth curves of non-genital organs according to age of worms.


Fig. 11
Growth curves of genital organs according to age of worms.

Tables


Table 1
Infection status of the loach, Misgurnus anguillicaudatus with the metacercariae of E. conetorchis


Table 2
Recovery rate of E. conetorchis form albino rate after experimental infection


Table 3
Measurement of E. conetorchis recovered from the experimentally infected albino rats


Table 4
Number and location of testes according to infection age of E. conetorchis in rats

References
1. Ando R, et al. Dobutsugaku Zasshi 1923;35:108–119.
2. Cho SY, Kang SY, Ryang YS. [Helminthes Infections In The Small Intestine Of Stray Dogs In Ejungbu City, Kyunggi Do, Korea]. Korean J Parasitol 1981;19(1):55–59.
 
3. Kawahara S, et al. Tokyo Iji Shinshi 1933;2840:1794–1796.
4. Komiya Y. Progress of Med Parasit in Jap 1965;2:1–328.
5. Okahashi K. Okayama Igakkai Zasshi 1966;78:15–24.
6. Saito S, et al. Japanese J Parasit 1982;31(4):291–287.
7. Seo BS, et al. Seoul J Med 1980;21(1):21–29.
8. Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies On Parasitic Helminths Of Korea 5.Survey On Intestinal Trematodes Of House Rats. Korean J Parasitol 1981;19(2):131–136.
 
9. Seo BS, Hong ST, Chai JY, Lee SH. Studies On Intestinal Trematodes In Korea: VIII. A Human Case Of Echinostoma Hortense Infection. Korean J Parasitol 1983;21(2):219–223.
 
10. Seo BS, Rim HJ, Lee CW. Studies on the parasitic helmiths of Korea: I. Trematodes of rodents. Korean J Parasitol 1964;2(1):20–26.
 
11. Takahashi S. Fukuoka Ika Daigaku Zasshi 1927;20(6):712–723.
12. Yakahashi S, et al. Tokyo Iji Shinshi 1930;2657:1326–1328.
13. Yakahashi S, et al. Tokyo Iji Shinshi 1930;2678:1326–1327.
14. s Tani.Japanese J Parasit 1976;25(4):262–273.
15. Yamashita J. Progress of Med Parasit in Jap 1964;1:287–313.
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2024 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI