Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Growth and development of Fibricola seoulensis metacercariae in tadpoles
| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasito Search

CLOSE

Korean J Parasito > Volume 24(2):1986 > Article

Original Article
Korean J Parasitol. 1986 Dec;24(2):109-114. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1986.24.2.109
Copyright © 1986 by The Korean Society for Parasitology
Growth and development of Fibricola seoulensis metacercariae in tadpoles
Soon Hyung Lee,Shon Moon Shin,Sung Tae Hong,Woon Mok Sohn,Jong Yil Chai and Byong Seol Seo*
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110, Korea.
*Department of Parasitology, College of Medicine, Inha University, Inchon, 160-01, Korea.
Abstract

In order to observe the growth and development of Fibricola seoulensis metacercariae, the tadpoles of Rana nigromagulata were experimentally infected with the cercariae. The metacercariae of various developmental stages were recovered from the tadpoles after 2 to 65 days of infection. They were prepared for morphological observation, and were given orally to mice to observe their infectivity. The following results were obtained. All of the tadpoles exposed to the cercariae were observed to harbour the larvae in their abdominal cavity. The young metacercariae of 2 days after infection were 121.1 µm long and 63.3 µm wide. They grow linearly for the first 14 days to be 262.0 µm long and 166.4 µm wide. Thereafter, no more growth recognized until 65 days. The larvae of 2 days old were similar with cercarial body and had 2 suckers, a pharynx, two ceca and a primordium of germ cells but no tribocytic organ. On the 8th day, they had tribocytic organ, and their morphology resembled that of mature metacercariae. The metacercariae younger than 10 days could not infect the mice. Only the metacercariae older than 14 days had infectivity. The recovery rate increased by the age of metacercariae from 19.0% in 14 days old to 70.0% in 40 days old. Above findings indicate that the tadpole is indispensible for metacercarial development and it needs at least 2 weeks for maturation. The tadpole is a pivotal host in the life cycle of F. seoulensis for connection between the snail and the frog.

Figures


Fig. 1
The growth curve of the metacercariae of Fibricola seoulensis recovered from the experimentally infected tadpoles by age.


Figs. 2-7
Fig. 2. A 2-day old metacercaria of F. seoulensis recovered from tadpoles, acetocarmine stained (scale; 50µm).

Fig. 3. A 3-day old metacercaria of F. seoulensis. acetocarmine stained (scale; 50µm).

Fig. 4. A 5-day old metacercaria of F. seoulensis. acetocarmine stained (scale; 50µm, GCM; germ cell mass).

Fig. 5. A 8-day old metacercaria of F. seoulensis. acetocarmine stained (scale; 50µm, OS; oral sucker, VS; ventral sucker, TO; tribocytic organ).

Fig. 6. A 14-day old metacercaria of F. seoulensis. acetocarmine stained (scale; 100µm).

Fig. 7. A metacercaria of F. seoulensis form the naturally infected snake, acetocarmine stained (scale; 100µm).


Tables


Table 1
Measurements of acetocarmine stained metacercariae of Fibricola seoulensis recovered from experimentally infected tadpoles (average of 10 metacercariae)


Table 2
The infectivity of Fibricola seoulensis metacercariae by age

References
1. Chandler AC. Trans Am Micr Soc 1942;61(2):156–167.
 
2. Cho SY, et al. Chung-Ang J Med 1983;8(1):12–27.
3. Cook TW. J Parasit 1978;64(5):938–939.
 
4. Hong SJ, et al. A case of Fibricola seoulensis and sparganum infection among snake eaters and follow up observation after treatment with praziquantel. Korean J Parasit 1985;23(2):362–363.
 
5. Hong ST, et al. Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus. Korean J Parasit 1984;22(1):61–65.
 
6. Hong ST, Cho TK, Hong SJ, Chai JY, Lee SH, Seo BS. Fifteen human cases of Fibricola seoulensis infection in Korea. Korean J Parasitol 1984;22(1):61–65.
 
7. Hong ST, Hong SJ, Lee SH, Seo BS, Chi JG. Studies On Intestinal Trematodes In Korea: VI. On The Metacercaria And The Second Intermediate Host Of Fibricola Seoulensis. Korean J Parasitol 1982;20(2):101–111.
 
8. Hong ST, Lee SH, Chai JY, Seo BS. [A study on the infection status of tadpoles and frogs by the metacercariae of Fibricola seoulensis in Korea]. Korean J Parasitol 1985;23(1):73–78.
 
9. Lee SH, Lee HJ, Hong ST, Huh S, Seo BS. The effect of temperature and salinity on maturation and hatching of Fibricola seoulensis eggs. Korean J Parasitol 1986;24(2):115–120.
 
10. Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies On Parasitic Helminths Of Korea 5.Survey On Intestinal Trematodes Of House Rats. Korean J Parasitol 1981;19(2):131–136.
 
11. Seo BS, Lee SH, Hong ST, Hong SJ, Kim CY, Lee HY. Studies On Intestinal Trematodes In Korea: V. A Human Case Infected By Fibricola Seoulensis (Trematoda: Diplostomatidae). Korean J Parasitol 1982;20(2):93–99.
 
12. Seo BS, Rim HJ, Lee CW. Studies on the parasitic helmiths of Korea: I. Trematodes of rodents. Korean J Parasitol 1964;2(1):20–26.
 
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2024 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI