Detection of circulating antigens in rats experimentally infected with <i>Paragonimus westermani</i> by ELISA

, Yong, Tai Soon; , Kim, Tong Soo; , Lee, Jong Soo; , Lee, On Young; , Kim, Dong Chan

doi:1987.25.2.141

Korean J Parasito > Volume 25(2):1987 > Article

Original Article


Korean J Parasitol. 1987 Dec;25(2):141-148. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1987.25.2.141
Copyright © 1987 by The Korean Society for Parasitology


Detection of circulating antigens in rats experimentally infected with Paragonimus westermani by ELISA
Tai Soon Yong,Tong Soo Kim,Jong Soo Lee,On Young Lee and Dong Chan Kim
Division of Parasitology, National Institute of Health, Seoul 122, Korea.


Abstract
Circulating antigens in rats experimentally infected with Paragonimus westermani were examined by ELISA. From a total of 22 albino rats, each fed with 25 metacercariae, blood samples were collected until 12 weeks after infection. The specific antibodies against P. westermani in the serum of an infected cat were purified by ammonium sulfate precipitation, DEAE anion-exchange chromatography and affinity chromatography serially. So-called double antibody sandwich ELISA method was used for the detection of circulationg antigens. The results were as follows: Mean value of O.D. in control sera was 0.04 (S.D.=0.04). After infection, mean O.D.(S.D) values were changed serially: 0.03(0.01) at 0.5 week(3 days), 0.55(0.50) at 1 week, 0.69(0.45) at 1.5week, 0.20(0.19) at 2 weeks and 0.13(0.10) at 2.5 weeks of infection. They returned, thereafter, to the level before infection. When 0.16(mean+3 S.D.) were considered as cut-off value, those higher than 0.16 were observed only in the sera collected between 1 and 2.5 weeks after infection. Average 8.4 immature worms (2.2 from the lungs and pleural cavities; 6.2 from muscles) were recovered in a rat at 12 weeks after infection. The fact that circulating antigens were not detected after 3 weeks of infection was considered to be caused by the formation of antigen-antibody complexs.

Figures


	Fig. 1 Changing pattern of circulating antigen levels presented by O.D. in albino rats each infected with 25 metacercariae of Paragonimus westermani.


	Fig. 2 The linear regression line of standard curve, representing circulating antigen levels, using P. westermani excretory-secretory antigens diluted in PBS, instead of rat test sera.


	Fig. 3 Changing pattern of circulating specific IgG levels presented by O.D. in albino rats each infected with 25 metacercariae of P. westermani.


	Fig. 4 An immature worm of P. westermani recovered from the lungs of a rat at 12 weeks after infection.


	Fig. 5 An immature worm of P. westermani recovered from the muscles of a rat at 12 weeks after infection.

Tables


	Table 1 Recovery of immature worms at 12 weeks after infection from 10 rats each infected with 25 metacercariae of P. westermani

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