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Korean J Parasitol > Volume 26(3):1988 > Article

Original Article
Korean J Parasitol. 1988 Sep;26(3):163-167. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1988.26.3.163
Copyright © 1988 by The Korean Society for Parasitology
Antigenic protein fractions reacting with sera of sparganosis patients
Sung Ho Choi,Shin Yong Kang,Yoon Kong and Seung-Yull Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Abstract

To observe the antigenic protein fractions in saline extract of Spirometra mansoni plerocercoid (sparganum), the crude extract was separated in reducing conditions of sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE). The proteins, transferred by electrophoresis to nitrocellulose paper, were reacted with sera from 15 surgically confirmed sparganosis and 24 cysticercosis patients for immunoblotting. Out of 30 identified protein bands in the extract, bands of 29 and 36 kilodaltons (kDa) were the strongest and the most frequently reacting with specific antibody (IgG) in sparganosis sera. Bands of higher molecular weight also reacted with the sera but their frequency of reactions was lower. Sera of cysticercosis reacted with different protein bands in saline extract of sparganum, but the cross reactions were observed in strong antigenic bands of 29 and 36 kDa.

Figures


Fig. 1
Findings of SDS-PAGE of saline extract of sparganum. SDS-PAGE was done in 10~15% linear gradient gel. The saline extract was heat-treated with sample buffer containing 10% 2-mercaptoethanol. Coomassie brilliant blue R-250 stained. Mr: Molecular weight in kDa.


Fig. 2
SDS-PAGE/immunoblot findings in 15 confirmed human sparganosis patients to saline extract of spargana. Mr: Molecular weight in kDa.


Fig. 3
SDS-PAGE/immunoblot findings in 24 confirmed neurocysticercosis to saline extract of spargana. Patients No. 1~6 and 23~24 cross-reacted positively by ELISA against sparganum antigen. Mr: Molecular weight in kDa.

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