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Korean J Parasitol > Volume 26(3):1988 > Article

Original Article
Korean J Parasitol. 1988 Sep;26(3):169-173. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1988.26.3.169
Copyright © 1988 by The Korean Society for Parasitology
Passive immunity by splenocyte transfer against amebic meningoencephalitis in mice
Kyung Il Im and Jae Sook Ryu
Department of Parasitology and Institute of Tropical Medicine, College of Medicine, Yonsei University, Seoul 120-752, Korea.
Abstract

The role of passive cell-mediated transfer of immunity against primary amoebic meningoencephalitis(PAME) in mice was studied. Naegleria fowleri, ITMAP 359, were cultured in CGVS medium. The ICR mice used were six week-old males of average weight of 15 g. Immunization was done by three intraperitoneal injections of l × 10(6) N. fowleri trophozoites at the interval of one week. Splenocytes were obtained from normal and immune mice spleens, and 1 × 10(7) cells were administered intraperitoneally into mice 3 days before challenge infection. Mice were infected intranasally with 7 × 10(4) N. fowleri trophozoites in a 3 µl suspension under secobarbiturate anesthesia. Transplants of normal or immune splenocytes seem to alter the pattern of the PAME development. The splenocytes transferred from immune mice reduced the mortality rate in the N. fowleri infected mice, as compared with the mice transferred with the same number of normal splenocytes or without splenocyte. The blastogenic response of the splenocytes to both lipopolysaccharide and concanavalin A was elevated on day 7 after infection the mice transinoculated with immune splenocytes. The serum antibody titers in the mice transferred with immune splenocytes were increased gradually from day 7 up to day 20 after infections by mean of ELISA. It is suggested that the transfer of splenocytes from immunized mice conferred immunity against N. fowleri infection.

Figures


Fig. 1
Survival rates of mice transinoculated with normal splenocytes (-•-•-•-), immune splenocytes (————), or no splenocytes (-------), and challenged with 7×104N. fowleri trophozoites.


Fig. 2
The stimulation index in LPS treated splenocyte cultures from mice transinoculated with normal splenocytes (-•-•-•), immune splenocytes (————), or no splenocytes (• • • • • • •), and challenged with 7×104N. fowleri trophozoites.


Fig. 3
The stimulation index in Con. A treated splenocyte cultures from mice transinoculated with normal splenocytes (-•-•-•), immune splenocytes (————), or no splenocytes (• • • • • • •), and challenged with 7×104N. fowleri trophozoites.


Fig. 4
Changes of circulating antibody (ELISA value) in mice transinoculated with normal splenocytes (-•-•-•-), immune splenocytes (————), or no splenocytes (• • • • •), and challenged with 7×104N. fowleri trophozoites.

Tables


Table 1
Cumulative numbers of death of mice infected with N. fowleri

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