Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Immunoblot observation of antigenic protein fractions in Paragonimus westermani reacting with human patients sera
| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasito Search

CLOSE

Korean J Parasito > Volume 26(4):1988 > Article

Original Article
Korean J Parasitol. 1988 Dec;26(4):239-243. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1988.26.4.239
Copyright © 1988 by The Korean Society for Parasitology
Immunoblot observation of antigenic protein fractions in Paragonimus westermani reacting with human patients sera
Sung Hwan Kim,Yoon Kong,Suk Il Kim,Shin Yong Kang and Seung-Yull Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Abstract

In order to observe the antigenic fractions in saline extract of adult Paragonimus westermani, proteins in the crude extract were separated by sodium dodecyl sulfate-polyacylamide gel electrophoresis (SDS-PAGE) in reducing conditions. The separated protein fractions were transferred to nitrocellulose paper on which 20 sera from human paragonimiasis were reacted and immunoblotted. Out of 15 stained protein bands in SDS-PAGE, 7 reacted with the sera. Of 14 reacted bands, 30 kilodalton(kDa) band was the most frequently reacted (95%) and was a strong antigen. Protein bands of 23 and 46 kDa were also strong antigens. Bands of over 150 kDa, 120 kDa, 92 kDa, 86 kDa, 74 kDa, 62 kDa, 51 kDa, 32 kDa, 28 kDa, 16.5 kDa and 15.5 kDa were also reactive but their frequencies of the reaction were variable.

Figures


Fig. 1
Finding of SDS-PAGE of saline extract of adult P. westermani. SDS-PAGE was done in 10~15% linear gradient gel. Antigen was heat-treated with sample buffer containing 10% 2-mercaptoethanol. Coomassie brilliant blue R-250 stained.

Mr: Molecular weight of marker proteins in kDa.

BPB: Dye front of bromphenol blue.



Fig. 2
SDS-PAGE/ immunoblot finding in 20 active human paragonimiasis patients.

Mr: Molecular weight in kDa.


References
1. Cho SY, Hong ST, Rho YH, Choi SY, Han YC. Application of micro-ELISA in serodiagnosis of Human paragonimiasis. Korean J Parasitol 1981;19(2):151–156.
 
2. Cho SY, Kang SY, Kim SI. Analysis of antigen specificity using monoclonal and polyclonal antibodies to Cysticercus cellulosae by enzyme-linked immunoelectrotransfer blot technique. Korean J Parasitol 1987;25(2):159–167.
 
3. Cho SY, Lee DK, Kang SY, Kim SI. [An Epidemiological Study Of Human Paragonimiasis By Means Of Micro-ELISA]. Korean J Parasitol 1983;21(2):246–256.
 
4. Choi WY, Yoo JE, Nam HW, Choi HR. [Purification of antigenic proteins of Paragonimus westermani and their applicability to experimental cat paragonimiasis]. Korean J Parasitol 1986;24(2):177–186.
 
6. Kim SI, Kang SY, Cho SY. [On The Applicability Of Partially Purified Antigenic Preparations Of Paragonimus Westermani]. Korean J Parasitol 1983;21(2):257–264.
 
7. Kojima S, et al. Jpn J Parasitol 1983;32:413–418.
8. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.
  
9. Lee OR, Chang JK. [ELISA of paragonimiasis in cat by crude and purified antigens of Paragonimus westermani]. Korean J Parasitol 1986;24(2):187–193.
 
10. Lee OR, Choi WY. [Comparison Of Agar-Gel Diffusion Tests, Counterimmunoelectrophoresis And Enzyme-Linked Immunosorbent Assay In The Sera Of Skin Test Positives For Paragonimiasis]. Korean J Parasitol 1983;21(2):270–280.
 
11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
 
13. Rim HJ, Chang YS, Lee JS, Joo KH, Suh WH, Tsuji M. Clinical Evaluation Of Praziquantel(Embay 8440; Biltricide(R)) In The Treatment Of Paragonimus Westermani. Korean J Parasitol 1981;19(1):27–37.
 
15. Sugiyama H, Sugimoto M, Akasaka K, Horiuchi T, Tomimura T, Kozaki S. Characterization and localization of Paragonimus westermani antigen stimulating antibody formation in both the infected cat and rat. J Parasitol 1987;73(2):363–367.
  
16. Tsang VC, Peralta JM, Simons AR. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol 1983;92:377–391.
  
17. Yokogawa M, et al. Jpn J Parasitol 1983;32:317–322.
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2024 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI