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Korean J Parasitol > Volume 27(1):1989 > Article

Original Article
Korean J Parasitol. 1989 Mar;27(1):35-40. Korean.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1989.27.1.35
Copyright © 1989 by The Korean Society for Parasitology
Observation of mucosal pathology after praziquantel treatment in experimental Fibricola seoulensis infection in rats
S H Lee,B I Kim,S T Hong,W M Sohn,* and J Y Chai
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-460, Korea.
Department of Parasitology*, College of Medicine, Inje University, Pusan 614-112, Korea.
Abstract

It is well known that the duodenum of mice or rats infected with Fibricola seoulensis shows atrophy of villi (shortening, blunting, widening, fusion) and hyperplasia of crypts. This study was performed to observe healing process of these pathological changes after deworming with anthelmintic treatment. Albino rats infected each with 1,000 metacercariae of F. seoulensis were treated with single dose of 10 mg/kg praziquantel on day 15 post-infection. On day 1, 3, 5, 7, 15, 21 and 28 after the treatment, they were sacrificed and their duodenums were histopathologically studied. Control (uninfected) rats showed their normal finger-like projections of duodenal villi and well arranged crypts. In comparison, untreated (infected) controls revealed severe mucosal changes characteristic of villous atrophy and crypt hyperplasia in their duodenum. The damaged duodenal mucosa was found to restore its normal morphology after praziquantel treatment; until day 3 post-treatment the mucosa was severely atrophied; on day 5 long and slender villi sometimes appeared among the fused and stout ones; after day 15 the villi were in their normalizing process. From this experiment, it was shown that the mucosal changes in the duodenum of rats caused by F. seoulensis infection were completely reversible in 21-28 days after anthelmintic treatment.

Figures


Fig. 1
Section of the duodenum of a control (uninfected) rat, showing well arranged villi and crypts with their normal shape. ×48.


Fig. 2
Section of the duodenum of an infected control rat, showing remarkably destroyed mucosa with severely deformed villi and hyperplastic crypts due to F. seoulensis infection. ×40.



Duodenal section of 1 day post-treatment group. ×48.


Fig. 4
Duodenal section of 5 day post-treatment group. ×48.


Fig. 5
Duodenal section of 15 day post-treatment group, showing villi and crypts restoring their normal configurations. ×48.


Fig. 6
Duodenal section of 28 day post-treatment group, revealing nearly normalized villi and crypts with high V/C ratio. ×56.

Tables


Table 1
Number of rats used in this study


Table 2
Histopathological findings of the duodenum of albino rats infected with F. seoulensis and changing patterns after treatment with praziquantel

References
1. Chai JY. Seoul J Med 1979;20(2):104–117.
2. Cho SY, et al. Chung-Ang J Med 1983;8(1):13–29.
3. Eastwood GL. Gastrointestinal epithelial renewal. Gastroenterology 1977;72(5 Pt 1):962–975.
 
5. Hong SJ, Lee SH, Seo BS, Hong ST, Chai JY. Studies On Intestinal Trematodes In Korea: IX. Recovery Rate And Development Of Fibricola Seoulensis In Experimental Animals. Korean J Parasitol 1983;21(2):224–233.
 
6. Hong ST. Studies On Intestinal Trematodes In Korea: VII. Growth, Development And Recovery Of Fibricola Seoulensis From Experimentally Infected Rats And Mice. Korean J Parasitol 1982;20(2):112–121.
 
7. Hong ST, Chai JY, Lee SH. Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus. Korean J Parasitol 1986;24(1):95–97.
 
8. Hong ST, Cho TK, Hong SJ, Chai JY, Lee SH, Seo BS. Fifteen human cases of Fibricola seoulensis infection in Korea. Korean J Parasitol 1984;22(1):61–65.
 
9. Kang SY, Cho SY, Chai JY, Lee JB, Jang DH. A Study On Intestinal Lesions Of Experimentally Reinfected Dogs With Metagonimus Yokogawai. Korean J Parasitol 1983;21(1):58–73.
 
10. Lee JB, Chi JG, Lee SK, Cho SY. Study On The Pathology Of Metagonimiasis In Experimentally Infected Cat Intestine. Korean J Parasitol 1981;19(2):109–129.
 
11. Lee SH, Chai JY, Seo BS. Studies on intestinal trematodes in Korea: XVIII. Effects of praziquantel in the treatment of Fibricola seoulensis infection in albino rats. Korean J Parasitol 1985;23(1):41–46.
 
12. Lee SH, Hong ST, Kim CS, Sohn WM, Chai JY, Lee YS. Histopathological changes of the liver after praziquantel treatment in Clonorchis sinensis infected rabbits. Korean J Parasitol 1987;25(2):110–122.
 
13. Lee SH, Yoo BH, Hong ST, Chai JY, Seo BS, Chi JG. A histopathological study on the intestine of mice and rats experimentally infected by Fibricola Seoulensis. Korean J Parasitol 1985;23(1):58–72.
 
15. Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies On Parasitic Helminths Of Korea 5.Survey On Intestinal Trematodes Of House Rats. Korean J Parasitol 1981;19(2):131–136.
 
16. Seo BS, Lee SH, Chai JY, Hong SJ, Hong ST. The life cycle and larval development of Fibricola seoulensis (Trematoda: Diplostomatidae). Korean J Parasitol 1988;26(3):179–188.
 
17. Seo BS, Lee SH, Hong ST, Hong SJ, Kim CY, Lee HY. Studies On Intestinal Trematodes In Korea: V. A Human Case Infected By Fibricola Seoulensis (Trematoda: Diplostomatidae). Korean J Parasitol 1982;20(2):93–99.
 
18. Seo BS, Rim HJ, Lee CW. Studies on the parasitic helmiths of Korea: I. Trematodes of rodents. Korean J Parasitol 1964;2(1):20–26.
 
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