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Korean J Parasitol > Volume 27(4):1989 > Article

Original Article
Korean J Parasitol. 1989 Dec;27(4):261-269. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1989.27.4.261
Copyright © 1989 by The Korean Society for Parasitology
The changes of histopathology and serum anti-sparganum IgG in experimental sparganosis of mice
S T Hong,K J Kim,*S Huh,Y S Lee,**J Y Chai,S H Lee and Y S Lee*
Department of Parasitology, Dermatology*, Forensic Medicine** and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-460, Korea.
Abstract

The present study is intended to observe the chronologic changes of experimental sparganosis by histopathological observation and detection of circulating anti-sparganum IgG antibody using ELISA. Each of 25 mice was infected with five spargana, and they were examined after 1, 2, 4, 10 weeks or 6 months from infection. The followings are summarized results. 1. The plerocercoids were detected in the subcutaneous tissue of the trunk, neck or axilla, but a few often extended into the skeletal muscle. The recovery rates were 72% at the first week, 80% at the second week, 95% at the fourth week, 92% at the tenth week and 100% at the sixth month. The larvae grew slowly in both length and weight until 6 months. 2. Histopathologically, most of the larvae were observed alive in the soft tissue or skeletal muscle. Numerous eosinophils, neutrophils, lymphocytes and plasma cells were infiltrated focally around the worms by the second week, but they surrounded the worms to form a layer of inflammatory reaction after 4 weeks of infection. Also histiocytes and fibroblasts began to appear around the inflammatory cells at 4 weeks. After 10 weeks, the worms encircled by a thin fibrous layer were found. After 6 months, the worms were surrounded by either fibrous tissue or active inflammatory cells. The inflammation looked more severe in the tracks left by the worms, rather than around the worms. 3. The level of anti-sparganum IgG antibody in the serum showed an increase by the fourth week, and a rapid and continuous increase was observed thereafter by the tenth week after infection.(ABSTRACT TRUNCATED AT 250 WORDS)

Figures


Figs. 1-6
Fig. 1. A sparganum in the subcutaneous tissue of a mouse(arrow head), 2 weeks after infection.

Fig. 2. A sectioned sparganum in the muscle fascia, 1 week after infection, ×40.

Fig. 3. A worm in a tunnel in the adipose tissue with focal infiltration of inflammatory cells, 2 weeks after infection, ×40.

Fig. 4. High power view of the focal inflammation, ×200.

Fig. 5. Massive infiltration of inflammatory cells in an empty tunnel in the muscle, 2 weeks after infection, ×40.

Fig. 6. The layer of fibroblasts around the worm, 4 weeks after infection, ×200.



Figs. 7-12
Fig. 7. Three sections of a sparganum in the subcutaneous tissue surrounded by inflammatory cells and fibrous tissue, 10 weeks after infection, ×40.

Fig. 8. The layer of foreign body giant cells and fibroblasts in the surrounding tissue, ×200.

Fig. 9. A sparganum in the muscle with severe inflammation, 6 months after infection, ×40.

Fig. 10. A sparganum in the subcutaneous daipose tissue surrounded by a thin layer of inflammatory cells and fibrous tissue, 6 months after infection, ×40.

Fig. 11. High power view of the surrounding tissue, 6 months after infection, ×200.

Fig. 12. A focus of cell infiltration, RBCs, eosinophils, lymphocytes and histiocytes, 6 months after infection, ×200.



Fig. 3
Anti-sparganum IgG antibody levels in serum by the duration of infection.

Tables


Table 1
Numbers and sites of spargana in experimental mice infected with five heads each


Table 2
The weights of spargana recovered from mice during the periods of infection


Table 3
Anti-sparganum IgG antibody levels in sera of mice during the periods of infection

References
1. Anegawa S, Hayashi T, Ozuru K, Kuramoto S, Nishimura K, Shimizu T, Hirata M. Sparganosis of the brain. Case report. J Neurosurg 1989;71(2):287–289.
  
2. Chang KH, Cho SY, Chi JG, Kim WS, Han MC, Kim CW, Myung H, Choi KS. Cerebral sparganosis: CT characteristics. Radiology 1987;165(2):505–510.
 
3. Chi JG, Chi HS, Lee SH. Histopathologic Study On Human Sparganosis. Korean J Parasitol 1980;18(1):15–23.
 
4. Cho SY, Bae JH, Seo BS. Some Aspects Of Human Sparganosis In Korea. Korean J Parasitol 1975;13(1):60–77.
 
5. Choi WJ. [Migration And Distribution Of Spargana In Body Of Experimentally Infected Mice]. Korean J Parasitol 1984;22(2):229–237.
 
6. Choi WY, Yoo JE, Nam HW, Choi HR. [Purification of antigenic proteins of Paragonimus westermani and their applicability to experimental cat paragonimiasis]. Korean J Parasitol 1986;24(2):177–186.
 
7. Fan KJ, Pezeshkpour GH. Cerebral sparganosis. Neurology 1986;36(9):1249–1251.
  
8. Hirai K, et al. Jpn J Parasitol 1987;36(6):405–409.
9. Hong ST, Lee SH. Histopathological and serological observations on experimental anisakiasis of rabbits. Korean J Parasitol 1987;25(2):168–180.
 
10. Horii Y, Owhashi M, Ishii A, Fujita K. Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid of Spirometra erinacei. J Helminthol 1989;63(1):6–12.
  
11. Ishii A. Jpn J Parasitol 1973;22(2):75–78.
12. Kim H, Kim SI, Cho SY. Serological Diagnosis Of Human Sparganosis By Means Of Micro-ELISA. Korean J Parasitol 1984;22(2):222–228.
 
13. Kittiponghansa S, Tesana S, Ritch R. Ocular sparganosis: a cause of subconjunctival tumor and deafness. Trop Med Parasitol 1988;39(3):247–248.
 
14. Lee SH, Chai JY, Seo BS, Cho SY. Two cases of human infection by adult of Spirometra erinacei. Korean J Parasitol 1984;22(1):66–71.
 
15. Min HK, Han SH, Yoon SO, Oh CH. [Intestinal Perforation Due To Infection Of Sparganum Mansoni]. Korean J Parasitol 1976;14(1):61–64.
 
16. Mueller JF. The biology of Spirometra. J Parasitol 1974;60(1):3–14.
 
17. Salem MA, Phares CK. Some biochemical effects of the growth hormone analogue produced by plerocercoids of the tapeworm Spirometra mansonoides on carbohydrate metabolism of adipose tissue from normal, diabetic, and hypophysectomized rats. J Parasitol 1986;72(4):498–506.
  
18. Suzuki N, et al. Jpn J Parasitol 1982;31(1):23–26.
19. Swartzwelder JC, Beaver PC, Hood MW. Sparganosis In Southern United States. Am J Trop Med Hyg 1964;13:43–47.
 
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