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Serum IgE levels in rats infected with Paragonimus westermani
M H Shin,J S Ryu and D Y Min
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
Abstract
Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was inoculated orally with 20-25 metacercariae of P. westermani from Cambaroides similis. Before and after infection (1, 2, 3, 4, 6, 8 weeks) of P. westermani, the blood was collected from the retro-orbital venous plexus of rats and kept serum at -70℃. Serum total IgE and specific IgG levels were determined by the capture and conventional enzyme-linked immunosorbent assay, respectively.
The results were as follows;
1. Serum IgE values were increased to 0.18 ± 0.042 at 2 weeks, 0.28 ± 0.151 at 4 weeks and 0.43 ± 0.055 at 8 weeks after infection. The absorbances of non-infected rats ranged 0.07 ± 0.021-0.12 ± 0.025.
2. Specific IgG values were slightly increased at 3 weeks (0.20 ± 0.032) and gradually increased up to 8 weeks (0.31 ± 0.067) after infection. The absorbances of non-infected rats ranged 0.11 ± 0.035-0.18 ± 0.019. The present results suggested that P. westermani could elevate serum IgE and specific IgG antibodies in Wistar rats which were not a good definitive host.
Tables
Table 1 Serum IgE values in rats infected with P. westermani
Table 2 Serum IgG values in rats infected with P. westermani
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