Production of interferon-γ and interleukin-4 by splenocytes in mice infected with <i>Paragonimus westermani</i>

, Shin, M H; , Min, D Y

doi:1996.34.3.185

Korean J Parasito > Volume 34(3):1996 > Article

Original Article


Korean J Parasitol. 1996 Sep;34(3):185-189. English. Published online Sep 20, 1996. http://dx.doi.org/10.3347/kjp.1996.34.3.185
Copyright © 1996 by The Korean Society for Parasitology


Production of interferon-γ and interleukin-4 by splenocytes in mice infected with Paragonimus westermani
M H Shin,^*¹ and D Y Min²
¹Department of Parasitology, College of Medicine, Ewha Womans University, Seoul 158-056, Korea.

Received May 11, 1996; Accepted July 31, 1996.
Abstract
The TH cytokine responses of spleen cells stimulated with Con A from mice infected with Paragonimus westermani were examined. The spleen cell culture supernatants were assayed for TH1-specific IFN-γ and TH2-specific IL-4. Cytokine responses for IL-4 peaked at three days (410 ± 60.9 pg/ml), persisted at a high level until the second week (343 ± 59.0 pg/ml), and then decreased slowly four and six weeks after infection. IFN-γ production by splenocytes only increased during the first week (151 ± 32.3 pg/ml) and declined abruptly after the second week of infection. IFN-γ production by splenocytes of infected mice was not observed during the sixth week of infection. In addition, serum IL-4 and IFN-γ were measured. Serum IL-4 was not detected in substantial quantity until four to six weeks after infection. The time course of serum IL-4 was not correlated with that of IL-4 production by splenocytes. Serum IFN-γ was undetectable during the entire course of infection. These results suggest that TH2 cytokine responses, rather than TH1, predominate in mice infected with P. westermani.

Tables


	Table 1 Cytokine levels (pg/ml) in splenocyte culture supernatant of mice infected with Paragonimus westermani


	Table 2 Serum cytokine level (pg/ml) in mice infected with Paragonimus westermani

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