<i>Toxoplasma</i> antibody titers by ELISA and indirect latex agglutination test in pregnant women

, Ryu, Jae Sook; , Min, Duk Young; , Ahn, Myoung Hee; , Choi, Han Gyoo; , Rho, Sang Chul; , Shin, Young Jeon; , Choi, Boyoul; , Joo, Hoo Don

doi:1996.34.4.233

Korean J Parasito > Volume 34(4):1996 > Article

Original Article


Korean J Parasitol. 1996 Dec;34(4):233-238. English. Published online Dec 20, 1996. http://dx.doi.org/10.3347/kjp.1996.34.4.233
Copyright © 1996 by The Korean Society for Parasitology


Toxoplasma antibody titers by ELISA and indirect latex agglutination test in pregnant women
Jae-Sook Ryu,¹Duk-Young Min,^*¹Myoung-Hee Ahn,¹Han Gyoo Choi,¹Sang Chul Rho,²Young Jeon Shin,²Bo youl Choi,² and Hoo Don Joo³
¹Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
²Department of Preventive Medicine, College of Medicine, Hanyang University, Seoul 133-791, Korea.
³National Veterinary Research Institute, RDA, Anyang 430-016, Korea.

Received November 28, 1996; Accepted December 02, 1996.
Abstract
The seroepidemiologic studies on anti-Toxoplasma antibody titers were carried out using ELISA and indirect latex agglutination test. Among 899 sera prepared from pregnant women, 39 cases (4.3%) revealed positive reaction and 218 sera from middle school students showed 4 positive reaction (1.8%) by ELISA. By LAT(newly established by National Veterinary Research Institute, Korea), the sera of 7 pregnant women (0.8%) showed positive reaction. When 80 sera showing ≥1:8 by LAT were used for comparing the results obtained from LAT and Toxotest-MT (Eiken Chemical Co., Japan), 7 cases and 8 sera were positive, respectively. All of 11 sera of proven toxoplasmosis patients showed positive reaction in both tests. Overall proportion of agreement between LAT kit and Toxotest-MT was 0.94(κ-index = 0.632, p<0.01), and LAT was considered to be useful for the screening of toxoplasmosis.

Figures


	Fig. 1 Comparison of positive rate between ELISA and LAT. ▪, 899 pregnant women; □, 218 middle school students In ELISA, cut-off absorbance was 0.25. In LAT, the positive border line was 1:64.


	Fig. 2 Age-specific Toxoplasma antibody positive rate of 39 pregnant women showed positive reaction by ELISA.

Tables


	Table 1 Age distribution of pregnant women


	Table 2 Comparison of frequencies of ILA titers between LAT and Toxotest-MT


	Table 3 Overall proportion of agreement between LAT and Toxotest-MT in 80 pregnant sera with titers over 1:8 by LAT


	Table 4 Overall proportion of agreement between IgG-ELISA and LAT in 899 sera of pregnant women

References


4.	Choi WY, Yoo JU, Kim WG. [Toxoplasma antibodies by indirect latex agglutination tests in St. Marys Hospital patients]. Korean J Parasitol 1982;20(1):33–37.

5.	Choi WY, Yoo JE, Chung CS, Paik KK, Cho SN. [Toxoplasma Antibodies By Indirect Latex Agglutination Tests In National Seoul Mental Hospital Patients]. Korean J Parasitol 1983;21(2):281–285.

6.	Choi WY, Choi HR, Rha JG. [Significance of Toxoplasma antibody titers by indirect latex agglutination tests in pregnant women and pelvic tumor patients. Korean J Parasitol 1985;23(2):300–304.

7.	Choi WY, Yoo JE, Nam HW, Oh CY, Kim SW, Katakura K, Kobayashi A. Toxoplasma antibodies by indirect latex agglutination tests in zoo animals. Korean J Parasitol 1987;25(1):13–23.

8.	Choi WY, Nam HW, Youn JH, Kim DJ, Kong Y, Kang SY, Cho SY. Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay. Korean J Parasitol 1992;30(2):83–90.

9.	Desmonts C, et al. Bull NY Acad Med 1974;50:146–159.

*10.*	Desmonts G, Couvreur J. Congenital toxoplasmosis. A prospective study of 378 pregnancies. N Engl J Med 1974;290(20):1110–1116.

*12.*	Ho-Yen DO, et al. Rev Med Microbiol 1990;1:229–235.

*13.*	Im KI, et al. Yonsei Rep Trop Med 1991;22:15–20.

*14.*	Joss AW, Skinner LJ, Chatterton JM, Chisholm SM, Williams HD, Ho-Yen DO. Simultaneous serological screening for congenital cytomegalovirus and toxoplasma infection. Public Health 1988;102(5):409–417.

*15.*	Kasper LH, Bradley MS, Pfefferkorn ER. Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol 1984;132(1):443–449.

*16.*	Kobayashi A, et al. Jpn J Parasitol 1977;26:175–180.

*17.*	Lunde MN, Jacobs L. Antigenic differences between endozoites and cystozoites of Toxoplasma gondii. J Parasitol 1983;69(5):806–808.

*18.*	Makioka A, Suzuki Y, Kobayashi A. Recognition of tachyzoite and bradyzoite antigens of Toxoplasma gondii by infected hosts. Infect Immun 1991;59(8):2763–2766.

*19.*	Remington JS. Toxoplasmosis in the adult. Bull N Y Acad Med 1974;50(2):211–227.

*20.*	Suh MD, et al. Korean J Vet Res 1993;33(4):623–632.

*21.*	Suh MD, et al. Korean J Vet Res 1995;35(3):583–593.

*22.*	Tsubota N, et al. Jpn J Parasitol 1977;26:286–290.

*23.*	Viens P, Auger P, Villeneuve R, Stefanescu-Soare I. Serological survey for congenital toxoplasmosis among 4,136 pregnant women. Trans R Soc Trop Med Hyg 1977;71(2):136–139.

*24.*	Bartlett A, Bidwell DE. Enzyme immunoassays for parasitic diseases. Trans R Soc Trop Med Hyg 1976;70(2):98–106.

*25.*	Wilson CB, Remington JS, Stagno S, Reynolds DW. Development of adverse sequelae in children born with subclinical congenital Toxoplasma infection. Pediatrics 1980;66(5):767–774.