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Korean J Parasitol > Volume 35(1):1997 > Article

Original Article
Korean J Parasitol. 1997 Mar;35(1):25-29. English.
Published online Mar 20, 1997.  http://dx.doi.org/10.3347/kjp.1997.35.1.25
Copyright © 1997 by The Korean Society for Parasitology
In vitro cultivation of Gymnophalloides seoi metacercariae (Digenea:Gymnophallidae)
J Kook,S H Lee and J Y Chai*
Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received February 24, 1997; Accepted February 28, 1997.

Abstract

Gymnophalloides seoi is a human intestinal trematode prevalent on southwestern islands in Korea. In the present study, we investigated whether G. seoi metacercariae can grow and develop into adults by in vitro cultivation. The metacercariae were obtained from naturally infected oysters, and cultured in vitro for 5 days under three conditions; 37℃/5% CO2, 41℃/8% CO2, or 41℃/5% CO2, in NCTC 109 complete media containing 20% FBS and 1% antibiotics-antimycotics. The degree of worm growth and development was compared with that grown in vivo of C3H mice. The length of the worms cultivated in vitro was 200-300 µm not significantly different from metacercariae, whereas the length of the worms recovered from C3H mice was significantly larger, 300-400 µm. The worms produced eggs when grown in C3H mice or cultured in vitro for 2 days under 41℃/8% CO2 or 41℃/5% CO2, but not when cultured under 37℃/5% CO2. Among the in vitro conditions, 41℃/5% CO2 was best for egg production, although the number of eggs was about half of worms obtained from C3H mice. In conclusion, in vitro cultivation of G. seoi metacercariae into egg-producing adults was partially successful under culture conditions of 41℃/5% CO2 or 41℃/8% CO2.

Figures


Fig. 1
The length growth (average values) of G. seoiin vivo and in vitro.


Fig. 2
Average number of uterine eggs in G. seoi grown in vivo and in vitro.

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