| Home | E-Submission | Sitemap | Contact us
top_img
Korean J Parasitol > Volume 55(2):2017 > Article
Kim, Han, Kim, Park, Min, Ahn, and Ryu: Interaction between Trichomonas vaginalis and the Prostate Epithelium

Abstract

Most men infected with Trichomonas vaginalis are asymptomatic and can remain undiagnosed and untreated. This has been hypothesized to result in chronic persistent prostatic infection. Adhesion of the protozoan organisms to mucosal cells is considered a first and prerequisite step for T. vaginalis infection. Adhesion of T. vaginalis to prostate epithelial cells has not yet been observed; however, there are several reports about inflammation of prostate epithelial cells induced by T. vaginalis. The aim of this study was to investigate whether adhesion and cytotoxicity of T. vaginalis are involved in inflammation of prostate epithelial cells. When RWPE-1 cells were infected with T. vaginalis (1:0.4 or 1:4), adhesion of T. vaginalis continuously increased for 24 hr or 3 hr, respectively. The cytotoxicity of prostate epithelial cells infected with T. vaginalis (RWPE-1: T. vaginalis=1:0.4) increased at 9 hr; at an infection ratio of 1:4, cytotoxicity increased after 3 hr. When the RWPE-1 to T. vaginalis ratio was 1:0.4 or 1:4, production of IL-1β, IL-6, CCL2, and CXCL8 also increased. Epithelial-mesenchymal transition (EMT) was verified by measuring decreased E-cadherin and increased vimentin expression at 24 hr and 48 hr. Taken together, the results indicate that T. vaginalis adhered to prostate epithelial cells, causing cytotoxicity, pro-inflammatory cytokine production, and EMT. Our findings suggest for the first time that T. vaginalis may induce inflammation via adhesion to normal prostate epithelial cells.

Trichomonas vaginalis, a parasitic protozoan, causes the sexually transmitted disease trichomoniasis, a common infection of the urogenital system [1]. The World Health Organization (WHO) estimated that over a half of the 276.4 million new T. vaginalis infections occur each year in men [2]. Because trichomoniasis is asymptomatic in over 75% of infected men, it can remain undiagnosed and untreated, which has been hypothesized to result in chronic persistent prostatic infection [3,4]. In addition, T. vaginalis has been detected recently in the urine of patients with prostatitis and in the prostate tissue of patients with benign prostatic hyperplasia or prostate cancer [57]. However, the host-parasite interaction of trichomoniasis remains incompletely understood in the prostate.
Adhesion of the organisms to mucosal cells is considered a first and prerequisite step for T. vaginalis infections [8,9]. When T. vaginalis was added to epithelial cells, the parasitic body changed to a more elongated shape with pseudopodia, showing high adherence to primary vaginal epithelial cells. In contrast, the morphology of T. vaginalis did not change when added to a cervical cancer epithelial cell line (HeLa) [10]. Therefore, T. vaginalis is able to recognize and specifically bind to host tissues. Adhesion of T. vaginalis to different cell types such as renal epithelial cells, colon epithelial cells, sperm, fibroblasts, leukocytes, or breast myocytes has been previously reported [1115]. However, adhesion of T. vaginalis to prostate epithelial cells has not yet been observed, although there are several reports on T. vaginalis-induced inflammation of prostate epithelial cells.
The aim of this study was to investigate whether adhesion and cytotoxicity of T. vaginalis could lead to inflammation of prostate epithelial cells. Our findings suggested that T. vaginalis adhered and caused cytotoxicity to prostate epithelial cells, with subsequent induction of inflammation and epithelial-mesenchymal transition (EMT).
T. vaginalis isolate T016 was grown in TYM media supplemented with 10% heat-inactivated horse serum. The human prostate epithelial cells (RWPE-1 cell line) were cultured in keratinocyte serum-free medium (K-SFM) containing 5 ng/ml human recombinant epidermal growth factor and 25 μg bovine pituitary extract supplemented with 5% FBS.
To confirm the adhesion of live T. vaginalis, RWPE-1 cells were incubated with T. vaginalis (RWPE-1: T. vaginalis=1:0.4 or 1:4) for 30 min, 3, 6, 9, 24, and 48 hr. T. vaginalis was stained with a CellTrackerOrange Fluorescent Probe (Invitrogen, Carlsbad, California, USA) according to the manufacturer’s instructions. Briefly, T. vaginalis was suspended in CellTrackerOrange solution, which was diluted to 0.5 μM in Hank’s solution (Invitrogen). After 30 min, the stained T. vaginalis was added to a monolayer of RWPE-1. After incubation, adherence of T. vaginalis was examined with a fluorescence microscope (Leica, Wetzlar, Germany), and images were analyzed by ImageJ.
To determine the effects of live T. vaginalis on the cytotoxicity of prostate epithelial cells, RWPE-1 cells were seeded on 96-well plates and cultured in complete K-SFM medium with 5% FBS. After 24 hr, the RWPE-1 medium was changed to serum-free RPMI1640 medium and incubated for 24 hr. T. vaginalis (RWPE-1:T. vaginalis=1:0.4 or 1:4) was then added into the RWPE-1 and incubated for various times. Cytotoxicity was analyzed using a CCK-8 assay. CCK-8 reagent (Enzo Life Sciences, Farmingdale, New York, USA) was added to each well for 2 hr prior to obtaining the spectrophotometric reading according to the manufacturer’s directions. The CCK-8 assay is a sensitive colorimetric assay to determine the number of viable cells in cell proliferation and cytotoxicity assays. The CCK-8 reagent is reduced by dehydrogenases, yielding a water-soluble formazan dye. The amount of dye generated is directly proportional to the number of living cells.
The production of inflammatory cytokines by RWPE-1 cells incubated with T. vaginalis (RWPE-1: T. vaginalis=1:0.4 or 1:4) for 9, 24, and 48 hr was measured using an ELISA for IL-1β, IL-6, CCL2, and CXCL8 (BD Biosciences, San Diego, California, CA). The data are presented as the mean±SD of 3 independent experiments.
To examine the epithelial-mesenchymal transition (EMT), expression of E-cadherin (epithelial marker), and vimentin (mesenchymal marker) was assessed using fluorescence microscopy. Briefly, RWPE-1 cells were grown on a glass coverslip in a 12-well plate (Corning) and incubated with T. vaginalis for 24 hr. The cells were fixed with 4% paraformaldehyde at −20°C for 10 min and blocked with 0.1% normal goat serum at room temperature for 1 hr. After being washed with PBS 3 times, the glass cover slips were incubated with anti-E-cadherin antibody (1:250, mouse monoclonal, Cell signaling) or anti-vimentin antibody (1:500, rabbit polyclonal, Abcam) overnight at 4°C. They were then washed and stained with CyTM3 AffiniPure donkey anti-mouse IgG (1:500, Jackson Immuno Research, West Grove, Philadelphia, USA) or Alexa 594-labelled goat anti-rabbit lgG (#A11012, 1:500, Invitrogen) at 37°C for 1 hr. The cover slips were mounted using Vectashield mounting medium (Vector Laboratories, Burlingame, California, USA) and stained with DAPI to visualize the nuclei. Fluorescence was then measured with a fluorescence microscope (Las Software, Leica).
Western blotting was also performed to confirm EMT. RWPE-1 cells were incubated with T. vaginalis (RWPE-1: T. vaginalis= 1:0.4) for 48 hr, separated by SDS–PAGE on a 12% polyacrylamide gel, and transferred to an Immun-Blot® PVDF membrane (Bio-Rad, Quarry Bay, Hong Kong). The membranes were probed with anti-E-cadherin antibody (1:1,000; Cell signaling) or anti-vimentin antibody (1:1,000; cell signaling) primary antibodies overnight at 4°C. The membranes were incubated with goat anti-rabbit IgG polyclonal antibody (1:10,000; ADISAB-300-J, Enzo Life Sciences) for 1 hr at room temperature. The blots were visualized using Chemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate (SurModics, Minneapolis, Minneapolis, USA). The signals were measured with a Chemi-Doc (Bio-Rad, Hercules, California, USA).
When prostate epithelial cells were reacted with T. vaginalis at a ratio of 1:0.4, adhesion of T. vaginalis began after 30 min and increased continuously for 24 hr. In contrast, at a ratio of 1:4, adherence of T. vaginalis to prostate epithelial cells peaked at 3 hr, after which the level sharply decreased (Fig. 1A, B). When RWPE-1 cells were infected with T. vaginalis (RWPE-1: T. vaginalis=1:0.4), the cytotoxicity of prostate epithelial cells increased gradually from 9 hr (2.0%) to 48 hr (26.4%). However, at a ratio of 1:4, the cytotoxicity of prostate epithelial cells increased from 3 hr to 48 hr, with a maximum level of 91.5% (Fig. 1C). In contrast to T. vaginalis-infected groups, cytotoxicity did not appear over a 48 hr period in the control group (RWPE only). This result was consistent with the results of Lustig et al. [16], who identified T. vaginalis contact-dependent cytolysis of epithelial cells. The 1:0.4 ratio showed higher adhesion and lower cytotoxicity at 24 hr. T. vaginalis is a microaerophilic anaerobic protozoa [17]. In contrast to the vagina, the prostate is assumed to be an anaerobic condition. In addition, high concentration of zinc in the prostate may induce a critical cytotoxicity to T. vaginal [18]. Considering that prostate tissue is a harsh condition for T. vaginalis survival, only a few T. vaginalis organisms remained viable in the tissue. Thus, 1:0.4 was chosen to determine cytokine levels and EMT.
To investigate the production of inflammatory cytokines by infected prostate epithelial cells, RWPE-1 cells were incubated with T. vaginalis (1:0.4) for 6, 24, and 48 hr. The RWPE-1 cells released the cytokines IL-1β, IL-6, CCL2, and CXCL8. Cytokine production was significantly greater in the infected group compared to the control group (Fig. 2). Production of IL-1β and CCL2 increased in a time-dependent manner (Fig. 2A, C), while the production of IL-6 and CXCL8 increased for 24 hr and subsequently decreased (Fig. 2B, D).
Several researchers have reported that the adhesion of T. vaginalis to vaginal epithelial cells plays an important role in the pathogenesis of trichomoniasis through production of CXCL8, CCL2, and IL-6 [9,19,20]. In previous studies, we reported that T. vaginalis-infected prostate epithelial cells produced pro-inflammatory cytokines such as IL-1β, IL-6, CCL2, and CXCL8 [21,22], which is consistent with the present results (Fig. 2). Therefore, these pro-inflammatory cytokines may be produced as a result of adhesion of T. vaginalis to prostate epithelial cells, which could promote the pathogenesis of prostatic disease. However, further study is required to determine whether inhibition of adhesion with anti-adhesin antibody or chemicals affect cytotoxicity and cytokine production. Also, considering invasiveness of T. vaginalis, it is interesting to examine whether adhesion of trichomonads to prostate stromal cells might be related to stromal inflammation or proliferation.
Expression of EMT markers was measured by fluorescence microscopy and western blot. In this study, we confirmed EMT through decreased expression of E-cadherin (an epithelial cell marker) and increased expression of vimentin (a mesenchymal cell marker) (Fig. 3A, B). EMT is a biological process that allows a polarized epithelial cell to undergo multiple biochemical changes enabling it to assume a mesenchymal cell phenotype. Enhanced migratory capacity, invasiveness, elevated resistance to apoptosis, and greatly increased production of extracellular matrix components occur in EMT [23]. EMT may be classified into 3 subtypes. Type 1 EMT involves the transition of primordial epithelial cells into motile mesenchymal cells and is associated with the generation of diverse cell types during embryonic development and organogenesis. Type 3 EMT occurs in carcinoma cells. Type 2 EMT involves transition of secondary epithelial cells to resident tissue fibroblasts and is associated with wound healing, tissue regeneration, and organ fibrosis [2325]. Also, type 2 EMT is induced in response to inflammation, but stops once inflammation is attenuated, especially during wound healing and tissue regeneration [24]. In the present study, EMT of prostate epithelial cells was induced by T. vaginalis at 24 and 48 hr, along with increased cytotoxicity and cytokine production. Thus, our results indicate that EMT may be classified as type 2, which suggests a healing process in wounded prostate epithelial cells.
The ability of epithelial cells to change morphology to more closely resembled mesenchymal cells is a feature of EMT. Tube formation in vascular endothelial cells observed in cancer tissues is indicative of metastasis [26]. Maniotis et al. [27] showed that vascular mimicry patterns form in vitro in the absence of hemodynamic forces, suggesting that the formation of a patterned non-endothelial cell-lined microcirculation may be an attribute of the tumor cell. In this study, tube-like formation was observed after 48 hr of incubation (Fig. 3C). Morphologic changes of prostate epithelial cells induced by EMT may lead to tube-like formation. We showed previously that T. vaginalis induced EMT in prostate epithelial cells [22]. In this study, we confirmed additional morphologic changes, as well as EMT.
In conclusion, adherence of T. vaginalis to prostate epithelial cells caused cytotoxicity, pro-inflammatory cytokine production, and EMT. Our findings suggested for the first time that T. vaginalis may induce inflammation via adhesion to normal prostate epithelial cells.

ACKNOWLEDGMENT

This research was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) (no. NRF-2009-0074788).

Notes

ETHICAL STANDARDS
The experiments complied with the current laws of this country.

CONFLICT OF INTEREST

CONFLICT OF INTEREST
The authors declare that they have no conflicts of interest.

REFERENCES

1. Abdolrasouli A, Amin A, Baharsefat M, Roushan A, Mofidi S. Persistent urethritis and prostatitis due to Trichomonas vaginalis: A case report. Can J Infect Dis Med Microbiol 2007;18: 308-310.
pmid pmc
2. World Health Organization. Global incidence and prevalence of selected curable sexually transmitted infections – 2008. Geneva, Switzerland. WHO. 2012, pp 1-20.

3. Seña AC, Miller WC, Hobbs MM, Schwebke JR, Leone PA, Swygard H, Atashili J, Cohen MS. Trichomonas vaginalis infection in male sexual partners: implications for diagnosis, treatment, and prevention. Clin Infect Dis 2007;44: 13-22.
crossref pmid
4. Sutcliffe S, Neace C, Magnuson NS, Reeves R, Alderete JF. Trichomonosis, a common curable STI, and prostate carcinogenesis-a proposed molecular mechanism. PLoS Pathog 2012;8: e1002801.
crossref pmid pmc
5. Lee JJ, Moon HS, Lee TY, Hwang HS, Ahn MH, Ryu JS. PCR for diagnosis of male Trichomonas vaginalis infection with chronic prostatitis and urethritis. Korean J Parasitol 2012;50: 157-159.
crossref pmid pmc
6. Mitteregger D, Aberle SW, Makristathis A, Walochnik J, Brozek W, Marberger M, Kramer G. High detection rate of Trichomonas vaginalis in benign hyperplastic prostatic tissue. Med Microbiol Immunol 2012;201: 113-116.
crossref pmid
7. Gardner WA Jr, Culberson DE, Bennett BD. Trichomonas vaginalis in the prostate gland. Arch Pathol Lab Med 1986;110: 430-432.
pmid
8. Alderete JF, Pearlman E. Pathogenic Trichomonas vaginalis cytotoxicity to cell culture monolayers. Br J Vener Dis 1984;60: 99-105.
pmid pmc
9. Alderete JF, Garza GE. Specific nature of Trichomonas vaginalis parasitism of host cell surfaces. Infect Immun 1985;50: 701-708.
pmid pmc
10. Kim SR, Ryu JS. Scanning electron microscopic observation of Trichomonas vaginalis contacted with human vaginal epithelial cells. Korean J Electron Microscopy 2001;31: 235-244.

11. Rendón-Maldonado JG, Espinosa-Cantellano M, González-Robles A, Martínez-Palomo A. Trichomonas vaginalis: in vitro phagocytosis of lactobacilli, vaginal epithelial cells, leukocytes, and erythrocytes. Exp Parasitol 1998;89: 241-250.
crossref pmid
12. Jesus JB, Vannier-Santos MA, Britto C, Godefroy P, Silva-Filho FC, Pinheiro AA, Rocha-Azevedo B, Lopes AH, Meyer-Fernandes JR. Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate. Parasitol Res 2004;93: 369-377.
crossref pmid
13. da Costa RF, de Souza W, Benchimol M, Alderete JF, Morgado-Diaz JA. Trichomonas vaginalis perturbs the junctional complex in epithelial cells. Cell Res 2005;15: 704-716.
crossref pmid
14. Benchimol M, de Andrade Rosa I, da Silva Fontes R, Burla Dias AJ. Trichomonas adhere and phagocytose sperm cells: adhesion seems to be a prominent stage during interaction. Parasitol Res 2008;102: 597-604.
crossref pmid
15. Vilela RC, Benchimol M. Trichomonas vaginalis and Tritrichomonas foetus: interaction with fibroblasts and muscle cells - new insights into parasite-mediated host cell cytotoxicity. Mem Inst Oswaldo Cruz 2012;107: 720-727.
crossref pmid
16. Lustig G, Ryan CM, Secor WE, Johnson PJ. Trichomonas vaginalis contact-dependent cytolysis of epithelial cells. Infect Immun 2013;81: 1411-1419.
crossref pmid pmc
17. Leitsch D. Recent Advances in the Trichomonas vaginalis Field. F1000Res 2016;5: 162.

18. Gimenes F, Souza RP, Bento JC, Teixeira JJ, Maria-Engler SS, Bonini MG, Consolaro ME. Male infertility: a public health issue caused by sexually transmitted pathogens. Nat Rev Urol 2014;11: 672-687.
crossref pmid
19. Kucknoor A, Mundodi V, Alderete JF. Trichomonas vaginalis adherence mediates differential gene expression in human vaginal epithelial cells. Cell Microbiol 2005;7: 887-897.
crossref pmid pmc
20. Alderete JF, Lehker MW, Arroyo R. The Mechanisms and Molecules Involved in Cytoadherence and Pathogenesis of Trichomonas vaginalis. Parasitol Today 1995;11: 70-74.
crossref
21. Seo MY, Im SJ, Gu NY, Kim JH, Chung YH, Ahn MH, Ryu JS. Inflammatory response of prostate epithelial cells to stimulation by Trichomonas vaginalis. Prostate 2014;74: 441-449.
crossref pmid
22. Han IH, Kim JH, Kim SS, Ahn MH, Ryu JS. Signalling pathways associated with IL-6 production and epithelial-mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis. Parasite Immunol 2016;38: 678-687.
crossref pmid
23. Kalluri R, Weinberg RA. The basics of epithelial-mesenchymal transition. J Clin Invest 2009;119: 1420-1428.
crossref pmid pmc
24. Lee K, Nelson CM. New insights into the regulation of epithelial-mesenchymal transition and tissue fibrosis. Int Rev Cell Mol Biol 2012;294: 171-221.
crossref pmid
25. Zeisberg M, Neilson EG. Biomarkers for epithelial-mesenchymal transitions. J Clin Invest 2009;119: 1429-1437.
crossref pmid pmc
26. Folberg R, Hendrix MJ, Maniotis AJ. Vasculogenic mimicry and tumor angiogenesis. Am J Pathol 2000;156: 361-381.
crossref pmid pmc
27. Maniotis AJ, Folberg R, Hess A, Seftor EA, Gardner LM, Pe’er J, Trent JM, Meltzer PS, Hendrix MJ. Vascular channel formation by human melanoma cells in vivo and in vitro: vasculogenic mimicry. Am J Pathol 1999;155: 739-752.
crossref pmid pmc

Fig. 1
Adherence of T. vaginalis to prostate epithelial cells (RWPE-1) and cytotoxicity of prostate epithelial cell. (A, B) T. vaginalis stained with CellTrackerOrange was added to a monolayer of prostate epithelial cells and was incubated for 48 hr. Orange colored T. vaginalis attached to prostate epithelial cells. Color intensity was measured by the ImageJ program. Bar=100 μm. (C) Cytotoxicity of prostate epithelial cells infected with T. vaginalis. Prostate epithelial cells were incubated with T. vaginalis at a ratio of 1:0.4 and 1:4 for 48 hr. Cell cytotoxicity was measured by CCK-8 assay.
kjp-55-2-213f1.gif
Fig. 2
Production of inflammatory cytokines by prostate epithelial cells stimulated with T. vaginalis (RWPE-1: T. vaginalis=1:0.4). When prostate epithelial cells were incubated with T. vaginalis for 9, 24, or 48 hr, prostate epithelial cells produced IL-1β (A), IL-6 (B), CCL2 (C), and CXCL8 (D). Each cytokine was measured by ELISA. IL-1β (A) and CCL2 (C) production increased in a time-dependent manner. *P<0.05 vs untreated RWPE-1. Statistical analyses were performed using SPSS statistical software, version 21 (IBM, Chicago, Illinois, USA). The Mann-Whitney U test was used to compare the results, and P-values<0.05 were considered statistically significant. The data are expressed as the mean±SD of 3–4 independent experiments.
kjp-55-2-213f2.gif
Fig. 3
Epithelial-mesenchymal transition (EMT) induced by prostate epithelial cells exposed to T. vaginalis (RWPE-1: T. vaginalis=1:0.4). (A, B) When prostate epithelial cells were infected with T. vaginalis for 48 hr, reduced E-cadherin (epithelial marker) expression and increased vimentin (mesenchymal marker) expression were observed by fluorescence microscopy after 24 hr (A) and by western blot after 48 hr (B). Bar=25 μm. (C) The morphologic changes of prostate epithelial cells started at 24 hr in response to T. vaginalis infection. Tube-like formation was observed at 48 hr. Bar=250 μm.
kjp-55-2-213f3.gif
Editorial Office
c/o Department of Parasitology and Tropical Medicine
Seoul National University College of Medicine Seoul 110-799, Korea
Tel : +82-2-740-8348   Fax : +82-2-765-6142   E-mail: cjy@snu.ac.kr, bono0988@naver.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2017 by The Korean Society for Parasitology and Tropical Medicine. All rights reserved.     powerd by m2community