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Korean J Parasitol > Volume 25(1):1987 > Article

Original Article
Korean J Parasitol. 1987 Jun;25(1):37-44. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1987.25.1.37
Copyright © 1987 by The Korean Society for Parasitology
Karyotype of Fasciola sp. obtained from Korean cattle
Jae Ku Rhee,Gil Soo Eun and Sang Bork Lee
Department of Veterinary Parasitology, Jeonbug National University, Jeonju 520, Korea.
Abstract

As a series of systematic classification for Korean common liver fluke, Fasciola sp., karyotype was investigated by means of the modified air-drying technique and of the regular Giemsa staining, Also, C-staining method was applied for detailed karyological analysis from the germ cells of the fluke. The following is a brief summary of the leading facts gained through the experiment. Korean Fasciola sp. was classified into three types based on their chromosomal complements; individuals with 20 or 30 chromosomes and with a 20/30 mosaic constitution. Worms having 30 chromosomes represent a triploid form with 3 sets of 10 basic chromosomes, while those with 20 chromosomes were diploid and mosaic individuals were 2n/3n mixoploid. The frequency of the individual type calculated is as follows; 67.45% of 212 flukes examined was of diploid, 10.85%, triploid, and the rest, 21.7%, mixoploid, respectively. In many cases, two or three types were found in the peculiar bovine host while single type inhabitant was about 20% out of 52 cases. The twenty chromosomes consisted of 1 parir of large metacentrics, 4 pairs of medium-sized subtelocentrics, and 5 pairs of small submetacentrics, while constitution of the thirty chromosomes was nearly interpreted as a triploid form with 3 sets of 10 basic chromosomes. The high centromeric indexes of both types are the first pairs among all the examined, and 37.93% was of diploid and 47.93%, triploid, respectively. In mixoploid individuals, constitution of the chromosomes of diploid or triploid cells was the same as that of diploid or triploid individuals. All the chromosomes of the germ cells in both types showed C-band around the centromeic region and especially the chromosomes no's 3, 7, and 8 showed a remarkable C-band distinguished from other chromosomes. The variance for the sizes of the worms and the eggs were not parallel with three different genotypes in Korean common liver fluke.

Figures


Fig. 1
The second meiotic metaphase chromosomes (10) of Fasciola sp. prepared by air-drying method.


Fig. 2
The mitotic metaphase diploid chromosomes (20) of Fasciola sp. prepared by air-drying method.


Fig. 3
The mitotic metaphase triploid chromosomes (30) of Fasciola sp. prepared by air-drying method.


Fig. 4
The mitotic metaphase mixoploid chromosomes (20) of Fasciola sp. prepared by air-drying method.


Fig. 5
The diploid chromosomes of Fasciola sp. prepared by air-drying and Giemsa staining method. A: Karyotypes of spermatogonia B: Karyotypes after C-staining method.


Fig. 6
The triploid chromosomes of Fasciola sp. prepared by air-drying and Giemsa staining method. A: Karyotypes of spermatogonia B: Karyotypes after C-staining method.

Tables


Table 1
Appearance rates of three types of chromosomes of Fasciola sp. in diferent Korean cattle


Table 2
Chromosome measurements and its classification of Fasciola sp.


Table 3
Comparative sizeds of worms and eggs among three types of Korean Fasciola sp.

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