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Korean J Parasitol > Volume 22(1):1984 > Article

Original Article
Korean J Parasitol. 1984 Jun;22(1):21-29. Korean.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1984.22.1.21
Copyright © 1984 by The Korean Society for Parasitology
Studies on intestinal trematodes in Korea X. Scanning electron microscopic observation on the tegument of Fibricola seoulensis
Byong Seol Seo,Soon Hyung Lee,Jong Yil Chai,Sung Tae Hong and Sung Jong Hong
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract

A scanning electron microscopic study was performed to observe the tegumental surface of adult Fibricola seoulensis. The adult worms were collected from the small intestine of mice 5 days to 3 weeks after experimental infection with the metacercariae. The metacercariae were obtained from the viscera of the snakes, Natrix tigrina lateralis, by artificial digestion technique. The results were as follows: The tegument of anterior body was covered with cobblestone-like cytoplasmic processes and that of posterior body showed finger-like processes. The posterior body had 4-5 large transverse wrinklings which formed many discontinued shallow rugae. The entire surface of anterior body was regularly arranged with the spines of which tips diverged into 3 to 4 points. They were densely packed in anterior mid-median portion of dorsal surface where appeared a few spines indented upto 5 points. Farther laterally and posteriorly from this portion, the pointed spines were more sparse and became single tipped and extended to anterior one-third of posterior body. The posterior surface of oral sucker was armed with 50-60 spines having 2-3 tips and ventral sucker also covered with such spines. On anteriormost dorsal surface arranged 60-70 spade-shaped spines. The tribocytic organ was armed with many stout recurved pile-like spines arranged radially. There were 3 types of sensory papillae. The ciliated knob-like (Type I) papillae were almost bilaterally symmetrical in ventral and dorsal surfaces of anterior body, and abundant especially around bases of oral and ventral suckers, tribocytic organ, and in lateral margins of anterior body. About 24 non-ciliated round swellings (Type II) were observed around each lip of oral and ventral suckers. The plate-1ike elevated papilla without cilium (Type III) was found to distribute only in posterior body. These 3 types of papillae seem to be tangoreceptive and/or rheoreceptive in function when their morphology and distributions are considered.

Figures


Diagram 1
Schematic drawing of tegumental differentiation of adult Fibricola seoulensis. (Left: dorsal surface, Right: ventral surface)


Figs. 1-6
*Scale unit is micrometer(µm)

Fig. 1. Ventral view of Fibricola seoulensis, showing bisegmented body and ventrally curved lateral margin of anterior body. Note tribocytic organ(TO) posterior to ventral sucker (VS). ×300.

Fig. 2. Ventral surface between ventral sucker and lateral margin, showing cobblestone-like cytoplasmic processes, spines(S) and knob-like (Type I) papilla(p) with a cilium(C). ×9,900.

Fig. 3. Anteriormost dorsal surface armed with spade-shape spines. ×20,600.

Fig. 4. Anterior odrso-median surface of anterior body, showing densely packed spines and scattered papillae (Type I: arrow). ×1,540.

Fig. 5. Higher magnification of Fig. 4. Note the spines diverged into 5 points. ×20,600.

Fig. 6. Dorso-mid-lateral surface of anterior body, showing more sparse armature and increased proportion of single tipped spines. ×15,300.



Figs. 7-12
*Scale unit is micrometer(µm)

Fig 7. Knob-like type I papilla with a cilium (C) found on the dorso-lateral surface of anterior body. ×30,600.

Fig. 8. Oral sucker with round swellings (Type II papillae: P) on the lip and spines on posterior surface. Note type I papillae (P1 ) around oral sucker. ×1,990.

Fig. 9. Higher magnification of the boxed area in Fig. 8. Note 2~3 pointed spines. x9,950.

Fig. 10. Tribocytic organ armed with stout recurved pile-like spines extruded from pits. x20,000.

Fig. 11. Finger-like cytoplasmic processes and plate-like elevation (Type III papilla: P) on a small wrinkling of posterior body. ×15,400.

Fig. 12. Anterior one-third of posterior body showing sparse distribution of single tipped spines. ×5,100.


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