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Korean J Parasitol > Volume 20(2):1982 > Article

Original Article
Korean J Parasitol. 1982 Dec;20(2):142-146. Korean.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1982.20.2.142
Copyright © 1982 by The Korean Society for Parasitology
Isolation of anthelmintic substance on Clonorchis sinensis from tuber of Bletilla striata
Jae Ku Rhee,Pyung Gil Kim,Byeong Kirl Baek,Sang Bork Lee and Byung Zun Ahn*
Department of Veterinary Medicine, Jeonbug National University, Korea.
*Department of Pharmacy, Chungnam National University, Korea.
Abstract

The present work was done to isolate the anthelmintic substance from the tuber of Bletilla striata which grows Gangweon-do of the Korea peninsula. The dried B. striata tuber was extracted with ethyl ether by a Soxhlet extractor at 40C for 24 hours, and then the extract was separated into insoluble material (negative wormicidal) and soluble material (positive wormicidal) by petroleum ether solvent. The wormicidal substance was separated in the silica gel column chromatography from the petroleum ether extract, using benzene (90 percent) and acetone (10 percent) as a solvent. The anthelmintic activity was observed in vitro on the cercaria, the excysted metacercaria and the adult of Clonorchis sinensis. On the silica gel thin layer chromatography with the wormicidal substance in benzene:acetone/90:10, five spots were observed in iodine chamber and the spot of Rf. 0.18 value of them appeared the most strong wormicidal effect on the cercaria of C. sinensis. Afterward, a substance which was equivalent to the Rf. 0.18 value spot was isolated from the wormicidal substance by means of silica gel column chromatography using 1 part of ethyl acetate and 6 parts of chloroform carrier, and the excysted matacercaria and the adult of C. sinensis were killed within 14 minutes and 128 minutes in the substance.

Figures


Fig. 1
Extraction method of anthelmintic substance from tuber of Bletilla striata on Clonorchis sinensis.

*: Wormicidal fraction (Bio-Test)

Ea: Ethyl acetate, C: Chloroform


Tables


Table 1
Anthelmintic effects of spots on the thin layer chromatography of the wormicidal extract from the tuber of Bletilla striata on Clonorchis sinensis cercariae


Table 2
Anthelmintic effects of fraction in the column chromatography of the wormicidal extract from the tuber of Bletilla striata on the excysted metacercaria and the adult of Clonorchis sinensis

References
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