Electron microscopical and histochemical studies on the epicuticle of <i>Echinorhynchus gadi</i> (Acanthocephala)

, Cho, Byung Chai

doi:1981.19.1.45

Korean J Parasito > Volume 19(1):1981 > Article

Original Article


Korean J Parasitol. 1981 Aug;19(1):45-54. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1981.19.1.45
Copyright © 1981 by The Korean Society for Parasitology


Electron microscopical and histochemical studies on the epicuticle of Echinorhynchus gadi (Acanthocephala)
Byung Chai Cho
Department of Parasitology, School of Medicine, Kyung Hee University, Korea.



Abstract
For the purpose of observing ultrastructure of the epicuticle of Echinorhynchus gadi, the present electron microscopical studies had been made. Also the histochemical methods of Morwy, Bauer, Smith, Lison, Taft, and those of lead and uranyl acetate had been used in order to see the distribution of glycogen, mucopolysaccharides, lipid and nucleic acid in the cuticle of Echinorhynchus gadi. The results obtained by the above studies were as follows: 1. Glycogen, mucopolysaccharides were found in the outermost, middle and inner layers, especially abundant in the middle layer of the cuticle. 2. Lipid was found in the middle and inner layer, and it was found abundantly around the lacunal canal in the cuticle. 3. Nucleic acid was found around the lacunal canal in the middle layer, and also distributed in the cell nucleus of inner layer in the cuticle. 4. Electron microscopically, the cuticle of Echinorhynchus gadi had three outer layers, being outermost, middle and inner ones. The outermost layer was medium electron dense, composed with plasmalemma and filaments. The middle layer was homogeneous one which was electron pale. The inner layer, which was electron dense, consisted of felt layer and radial layer. The electron dense glycogen, lipid granules were distributed in radial layer.

Figures


	Fig. 1 Integument showed outer, middle and inner layers, which were stained with light blue. Every section had been with alcian blue. ×450


	Fig. 2 Integument showed red purple, when it was stained with P.A.S. ×450


	Figs. 3 Integument showed light blue and parenchymal tissue show pink, when they were stained with nile blue. ×40


	Fig. 4 Outermost layer showed light blue, and middle and inner layers showed reddish pink, when they were stained with sudan black B. ×450


	Fig. 5 Epicuticle showed three layers: (1) Outermost layer was electron dense; (2) Middle layer showed electron pale with electron dense granules; (3) Inner layer showed electron dense with plasmalemma and filaments. ×6,250


	Fig. 6 The same as the above. ×15,000


	Fig. 7 Electron dense granule showed in the middle and inner layers. ×10,000


	Fig. 8 Subcuticle of Echinorhynchus gadi. ×6,250

Tables


	Table 1 Distribution of carbohydrate, lipid, and nucleic acid in cuticle of Echinorhynchusgadi

References


1.	Barabashova V. Parasiologiya 1972;5:446–454.

2.	Bullock WL. Histochemical studies on the Acanthocephala; the distribution of lipase and phosphatase. J Morphol 1949;84(2):185–199.

3.	Bullock WL. Histochemical studies on the Acanthocephala; the distribution of glycogen and fatty substances. J Morphol 1949;84(2):201–225.

4.	Chu JK, Kang SY, Chu JP, Sung DW. [Histochemical studies on Echinorhynchus gadi (Acanthocephala)]. Korean J Parasitol 1977;15(1):36–42.

5.	Crompton D. Parasitology 1963;54:663–685.

6.	Crompton DW, Lee DL. The Fine Structure Of The Body Wall Of Polymorphus Minutus (Goeze, 1782) (Acanthocephala). Parasitology 1965;55:357–364.

7.	Fukui T, et al. Jikken Igaku Zasshi 1937;21:36–41.

8.	Lee DL. The structure and composition of the helminth cuticle. Adv Parasitol 1966;4:187–254.

9.	Lee DL. The structure of the helminth cuticle. Adv Parasitol 1972;10:347–379.

*10.*	Lumsden RD. Surface ultrastructure and cytochemistry of parasitic helminths. Exp Parasitol 1975;37(2):267–339.

*11.*	Van Cleave HJ. J Parasit 1936;22:202–206.

*12.*	Van Cleave HJ. Experimental Parasit 1951;1:305–330.

*13.*	Von Brand T. J Parasit 1940;20:301–307.

*14.*	Von Brand T, et al. J Parasit 1942;28:315–318.

*15.*	Whitefield P. Parasitology 1971;63:49–58.

*16.*	Wright R, et al. J parasit 1968;54:1111–1123.

*17.*	Yamaguti S. Jpn J Zool 1939;8(3):318–351.