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A study on the lipids of Chinese liverfluke, Clonorchis sinensis
Soon-Hyung Lee,Chul Yong Song and Byong Ha Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.
Abstract
The present study was undertaken to observe the quality and quantity of lipids in the adult worms of Chinese liverfluke, Clonorchis sinensis.
Lipid extraction was done by the methods of Folch et a1. (l957) and Kenny (1952), and then the extracted lipid fractions of the worm were separated by thin layer chromatography. Those fractions were also subjected to perform the quantitative analyses of glycerides, cholesterols and phospholipids.
The results obtained were summarized as follows.
1. Total amount of glyceride was 37.56 mg per gram of worm tissue and the amount of monoglyceride was 8.34 mg per gm; diglyceride, 15.46 mg per gm; and triglyceride, 12.86 mg per gm.
2. Total amount of cholesterol was 3.30 mg per gm of worm tissue, and the esterified cholesterol (1.72 mg/gm) was a little more than that of free cholesterol (1.26 mg/gm).
3. The following 8 phospholipids were detected in the worm tissue of C. sinensis, i.e., lysophosphatidylcholine, phosphatidylcholine, phophatidylinositol, sphingomyelin, phosphatidylglycerol, phosphatidylserine, phosphatidylethanolamine and an unknown phospholipid.
Figures
Fig. 1 Thin layer chromatogram of phospholipid fractions of Clonorchis sinensis.
Table 2 Cholesterol contents in Clonorchis sinensis
Table 3 Phospholipid contents in Clonorchis sinensis
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