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Experimental study on the effect of cortisone in mice infected with Toxocara canis: Histopathological findings of granuloma in the liver
Keun-Tae Lee and Hong-Ki Min
Department of Parasitology, College of Medicine, Yonsei University, Korea.
Department of Parasitology, College of Medicine, Ewha Womans University, Korea.
Abstract
The present study was carried out to examine the effect of "Cortisone" on the worm burden migrated in the organs, and granulomatous change in the liver of mice infected with Toxocara canis eggs. Daily does of 0.5 mg "Cortisone" was administrated subcutaneously to Toxocara-infected mice with different schedule.
The results are summarized as follows;
1. More number of Toxocara larvae migrated into the muscle tissue, especially into the carcass through the wall of the large intestine and higher distribution rate and longer persistence of worms were also recognized in the carcass of the experimental group than in the control.
2. Cortisone administration resulted the inhibition of granuloma formation, as well as the resolution of granuloma in the liver. However, the discontinuation of the administration induced the reccurence of heavier grade of inflammation. In non-cortisone treated group (control), cellular infiltration appeared around the worms in the liver on the 10th day of the infection, and typical granulomatosis was followed on the 20th day.
As in above results, it is considered cortisone has remarkable anti-inflammatory and immunosuppressive effects in mice infected with T.canis, but histopathological changes on the granuloma formation might be related closely with the duration of Toxocara infection, dosage, duration and timing of cortisone administration.
Figures
Fig. 1 No inflammatory response around larva in the mouse liver. control, 5th day after infection, × 400
Fig. 2 A typical granuloma. control, 20th day after infection, × 100
Fig. 3 Resolved granuloma with localized cellular response. Group II, Cortisone treatment for 20 days since the day after infection, × 100
Fig. 4 Recurred granuloma. Group II, 5th day after discontinuation of cortisone treatment, × 100
Fig. 5 Confluent granulomas with eosinophilic abscess and extensive cellular infiltration. Group II, 20th day after discontinuation of cortisone treatment, × 100
Fig. 6 Moderately resolved granuloma with central necrosis. Group III, cortisone treatment for 10 days since 11th day after infection, × 400
Fig. 7 Granuloma with central eosinophilic abscess and necrosis. Group III, 15th day after discontinuation of cortisone treatment, × 100
Fig. 8 Resolving granuloma with central necrosis. Group IV, cortisone treatment for 5 days since 21th day after infection, × 100
Tables
Table 1 Distribution rates* of Toxocara canis larvae in the tissues of infected mice at various periods after infection.
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