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Korean J Parasitol > Volume 7(1):1969 > Article

Original Article
Korean J Parasitol. 1969 Jun;7(1):43-47. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1969.7.1.43
Copyright © 1969 by The Korean Society for Parasitology
Studies on lactic dehydrogenase activities in developmental stages of fertilized eggs of Ascaris lumbricoides
Chung Hee Lee, M.D.
Department of Parasitology, College of Medicine, Yonsei University, Korea.
Abstract

The lactic dehydrogenase activities of Ascaris lumbricoides have been assayed according to the developmental course of the fertilized eggs compared with the unfertilized eggs. Experimental procedures were modified that of Wroblewski and La Due's method with use of diphosphopyridine nucleotide reduced form(DPNH).

Sodium pyruvate and lactic dehydrogenase, the specific activities were expressed as DPNH unit per mg protein by method of Kornberg's. The experimental results are as follaws:

1. Lactic dehydrogenase activity in fertilized eggs of Ascaris are significantly increased from morula stage and its optimal pH is in 3.4 degrees. While any activities were not observed in unicell stage of fertilized eggs and unfertilized eggs.

2. Water soluble protein of fertilized eggs of Ascaris were varied in their amount during their developmental process. The amont of protein in tadpole stage eggs have increased to 1.3 times more than that of cleavage stage eggs.

3. However the amounts of water soluble protein in unfertilized eggs are revealed to one-third less than that of fertlized eggs.

4. The specific activity of lactic dehydrogenase in tadpole stage eggs are decreased to one third less than those of morula stage eggs during development, the stage is considered to be as a growth criteria in developmental process, as on this point the activity of aerobic cytochrome c oxidase and cyclophorase begin to increase respectively.

Figures


Fig. 1
Lactic dehydrogenase activity of developing Ascaris eggs in various pH ranges.


Fig. 2
Changes in Lactic dehydrogenase activity during development of Ascaris eggs.

Remarks: 1st group: unfertilized eggs, 2nd group: fertilized eggs, 3rd group: morula stage eggs, 4th group: tadpole stage eggs

Optimum conditions of enzymic reactions are held in PH 3.4 and at 24℃.



Fig. 3
Calibration curve of standard protein (serum albumin) with Folin-Ciocalteu's reagent.

Tables


Table 1
The amount of soluble protein in developing Ascaris eggs

Remarks:

  1. 1.0ml of supernatant was homogenated with water to 50% v/v and was put to analysis.

  2. Serum albumin (von Rind Germany) was used as the standard protein.


References
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