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Korean J Parasitol > Volume 3(3):1965 > Article

Original Article
Korean J Parasitol. 1965 Dec;3(3):122-126. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1965.3.3.122
Copyright © 1965 by The Korean Society for Parasitology
Metabolism of C14-acetate by cestodes
Han Jong Rim,Chung Jai Park,Yong Ok Min,Byong Jong On,Hyun Kyo Lee and Myong Soon Yun
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Department of Physiology, College of Medicine, Seoul National University, Korea.
Abstract

The adult worm and plerocercoid larva(sparganum) of Diphyllobothrium mansoni and Moniezia expansa employed in this experiment. The adult worms were divided into three portions, i.e. immature, mature and gravid proglottids, and each proglottids were incubated in 50 cc or 250 cc volume of special incubation flasks with incubation medium consisting of 10 cc of 25 cc of Krebs-Ringer phosphate buffer (pH 7.4). The incubation medium was added C14-acetate and non-radioactive carrier Na-acetate so as to contain acetate concentration of 50 mg per cent. The worms were allowed to incubate for 5 hours in the Dubnoff metabolic shaking incubator at 38℃.

After incubation period, the lactate and pyruvate appearance rate, total CO2 production tate, the turnover rates were employed as pervious report(Seo et al., 1965).

The quantitative analysis of C14-acetate utilized by the adult worm and plerocercoid larva of D. mansoni and M. expansa were compared and discussed in this report.

According to these data of the experiment, it is impressed that the fatty acid such as acetate may play a role of major part of their metabolism in the adult worm and plerocercoid larva of D. mansoni, whereas minor part of acetate participated in the metabolism by M. expansa.

Tables


Table 1
The oxidation metabolism of C14-acetate into respiratory CO2, lactate and pyruvate by plerocercoid larva and adult of Diphyllobothrium mansoni.


Table 2
The oxidation metabolism of C14-acetate into respiratory CO2, lactate and pyruvate by Moniezia expansa.


Table 3
The anaerobic metabolism of C14-acetate into glycogen by plerocercoid larva and adult of Diphyllobothrium mansoni.


Table 4
The anaerobic metabolism of C14-acetate into glycogen by Moniezia expansa.

References
1. Lee SI. [Metabolism Of C(14)-Lactate By Fasciola Hepatica And Eurytrema Pancreaticum]. Korean J Parasitol 1965;3(1):10–18.
 
2. Rim HJ. Seoul J Med 1963;4(2):133–142.
3. Rim HJ, Kim KS, Seong SH, Rhee SD, On BJ, Lee HK. [Metabolism of C(14)-glucose by Ascaridia galli]. Korean J Parasitol 1965;3(3):107–111.
 
4. Rim HJ, Seong SH, Park CJ, Rhee SD, Rhee SD, Lee HK. [Metabolism of C(14)-glucose by Moniezia expansa and Diphyllobothrium mansoni]. Korean J Parasitol 1965;3(3):112–116.
 
5. Seo BS, Rim HJ, Kim KS, Rhee SD, Lim SJ, Lee WS. Metabolism Of C(14)-Glucose By Fasciola Hepatica. Korean J Parasitol 1964;2(3):170–174.
 
6. Seo BS, Rim HJ, Kim KS, Lee MS, Kim YU, Song HY. Metabolism Of C(14)-Glucose By Eurytrema Pancreaticum. Korean J Parasitol 1964;2(3):175–178.
 
7. Seo BS, Rim HJ, Lee SI, Rhee SD, Lee WS, Lee JR. Metabolism Of C(14)-Glucose By Plerocercoid Of Diphyllobothrium Sp. Korean J Parasitol 1965;3(1):1–4.
 
8. Seo BS, Rim HJ, Lee SI, Park DK, Moon SC. [Metabolism of C(14)-glucose by Paramphistomum cervi]. Korean J Parasitol 1965;3(1):5–9.
 
9. Seo BS, Rim HJ, Min YO, Rhee SD, Lee TH, Yun MS. [Metabolism of C(14)-acetate by some trematodes]. Korean J Parasitol 1965;3(3):117–121.
 
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