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Korean J Parasitol > Volume 3(3):1965 > Article

Original Article
Korean J Parasitol. 1965 Dec;3(3):112-116. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1965.3.3.112
Copyright © 1965 by The Korean Society for Parasitology
Metabolism of C14-glucose by Moniezia expansa and Diphyllobothrium mansoni
Han Jong Rim,Soo Hyun Seong,Chung Jai Park,Sang Don Rhee,Byong Jong Oh and Hyu Kyo Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Department of Physiology, College of Medicine, Seoul National University, Korea.
Abstract

The adult worms of cestodes, Moniezia expansa and Diphyllobothrium mansoni employed in this experiment. The worms were divided into three portions, i.e. immature , mature and gravid proglottids, and each proglottids were incubated in a certain incubation period, and the glucose uptake rate, total CO2 production rate, tissue concentration and their radioactivities were employed as previous reports(Rim et al., 1965). The glucose uptake rate by M. expansa was a mean value of 6.46±1.23 micromole per hour per gram of wet wt. and the rate by D. mansoni was a mean value of 18.8±0.8 µM per hour per gram of wet wt. The higher rates were observed in the mature proglottid of M. expansa and in the immature proglottid of D. mansoni. The total CO2 production rates by the worms averaged 14.0±2.37 µM per hour per gram in M. expansa and 17.51±1.54 µM per hour per gram of wet wt. The relative specific activities of respiratory CO2(R.S.A CO2) averaged 22.2±5.15 percent in M. expansa and 54.2±2.2 per cent in D. mansoni. In the both worms, the higher values were obtained in the mature proglottids. Therefore, the average value of 8.84±2.66 per cent of glucose utilized by M. expansa and 8.23±0.50 percent of glucose utilized by D. mansoni from the medium glucose was oxidized into respiratory CO2. The tissue concentrations of glycogen were a mean of 2.21±0.46 percent per gram of wet wt. in M. expansa and 7.56±1.24 percent per gram of wet wt. in D. mansoni. The higher concentration of glycogen was observed in the gravid proglottids of M. expansa, however the gravid proglottids of D. mansoni showed lower concentration of glycogen than the other proglottids. The turnover rate of glycogen pool yielded with a mean of 0.04±0.01 miligram per hour per gram of wet wt. of M. expansa, whereas a mean of 1.66± 0.46 miligram per hour per gram wet wt. of D. mansoni. Therefore, a mean value of 2.58±0.93 per cent(R.G.D gly) of glucose utilized by M. expansa and 53.6±1.4 percent by D. mansoni was incorproated into the glycogen . These data account for that at least 11.42 per cent of the utilized glucose by M. expansa and 61.83 per cent of the utilized glucose by D. mansoni participated in furnishing the oxidation into respiratory CO2 and the synthetic process into glycogen.

Tables


Table 1
The oxidation metabolism of C14-glucose into respiratory CO2 by Moniezia expansa.


Table 2
The oxidation metabolism of C14-glucose into respiratory CO2 by Diphyllobothrium sp.


Table 3
The anaerobic metabolism of C14-glucose into glycogen by Moniezia expansa.


Table 4
The anaerobic metabolism of C14-glucose into glycogen by Diphyllobothrium sp.

References
1. Read CP. Carbohydrate metabolism of Hymenolepis diminuta. Exp Parasitol 1956;5(4):325–344.
  
2. Rim HJ. Seoul Jour Med 1963;4(2):133–142.
3. Rim HJ, Kim KS, Seong SH, Rhee SD, On BJ, Lee HK. [Metabolism of C(14)-glucose by Ascaridia galli]. Korean J Parasitol 1965;3(3):107–111.
 
4. Seo BS, Rim HJ, Kim KS, Rhee SD, Lim SJ, Lee WS. Metabolism Of C(14)-Glucose By Fasciola Hepatica. Korean J Parasitol 1964;2(3):170–174.
 
5. Seo BS, Rim HJ, Kim KS, Lee MS, Kim YU, Song HY. Metabolism Of C(14)-Glucose By Eurytrema Pancreaticum. Korean J Parasitol 1964;2(3):175–178.
 
6. Seo BS, Rim HJ, Lee SI, Rhee SD, Lee WS, Lee JR. Metabolism Of C(14)-Glucose By Plerocercoid Of Diphyllobothrium Sp. Korean J Parasitol 1965;3(1):1–4.
 
7. Seo BS, Rim HJ, Lee SI, Park DK, Moon SC. [Metabolism of C(14)-glucose by Paramphistomum cervi]. Korean J Parasitol 1965;3(1):5–9.
 
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