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Korean J Parasitol > Volume 2(3):1964 > Article

Original Article
Korean J Parasitol. 1964 Dec;2(3):159-164. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1964.2.3.159
Copyright © 1964 by The Korean Society for Parasitology
Studies on the metabolism of C14-proline in some parasitic helminths
Joo Soo Yoon
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract

A comparison of the absorption and incorporation of C14-proline into protein by the 7 kinds of helminth parasites is presented.

The radioactivity of free amino acid fraction is greater than that of protein fraction in all the worms, and only a small amount of exogenous labeled proline is incorporated into tissue protein.

In general, the pattern of C14-proline uptake and its incorporation into protein shows rapid linear increase during the period of 15 to 30 min and reaches the maximum at 60 min after incubation, and then the equilibrium state was maintained throughout further incubation.

Figures


Fig. 1
The free amino acid fraction by the various helminth parasites.

Total C14-activity/ medium:0.5 µC

Weight of carrier proline: 400 µg.

Temperature incubated: 37℃



Fig. 2
The Incorporation of C14-proline into protein fraction by the various helminth parasites at 37℃


Fig. 3
The uptake of C14-labeled proline and its incorporation into protein in Clonorchis sinensis after 15 min period of incubation at 37℃

Tables


Table 1
The uptake and incorporation of C14-proline by various worm parasites at 37℃, 60 min.

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