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Korean J Parasitol > Volume 2(1):1964 > Article

Original Article
Korean J Parasitol. 1964 Jun;2(1):91-96. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1964.2.1.91
Copyright © 1964 by The Korean Society for Parasitology
Paper chromatographic study on the amino acids of some parasitic helminths
Soon Hyong Lee,Byong Seol Seo and Joo Soo Yoon
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract

1) Unidimensional and two dimensional paper choromatogram were prepared of 10 kinds of parastic helminths.

2) Fourteen amino acids were identified from the acid hydrolysed tissue proteins of A. lumbricoides(cuticle and musculature), A. galli, F. hepatica, E. pancreaticum, P. cervi, T. solium, and M. expansa. They were glycine, alanine, serine, threonine, methione, valine, leucine, aspartic acid, lysine, arginine, tyrosine, proline and histidine. In hydrolysates of A. lumbricoides(female genital organ) and C. sinensis, 13 amino acids were recovered. Twelve amino acid from A. lumbricoides(intestinal tract), 9 from P. westermani, and 6 from H. nana were also identified in the tissue hydrolysates.

Figures


Fig. 1
Position of 14 amino acids identified from the acid hydrolysed tissue protein of F. hepatica after two dimensional chromatography.

Asp: Aspartic acid, Arg: Arginine, Ala: Alanine, Glu: Glutamic acid, Gly: Glycine, His: Histidine, Leu: Leucine, Lys: Lysine, Met: Methionine, Pro: Proline, Ser: Serine, Thr: Threonine, Tyr: Tyrosine, Val: Valine.


Tables


Table 1
Amino acids isolated from various worms.

References
1. Aldrich DV, Chandler AC, Daugherty JW. Intermediary protein metabolism in helminths. II. Effect of host castration on amino acid metabolism in Hymenolepis diminuta. Exp Parasitol 1954;3(2):173–184.
  
2. Ando T. Acta Mdica 1957;27:2342–2359.
4. Bird AF. Chemical composition of the nematode cuticle;observations of the whole cuticle. Exp Parasitol 1956;5(4):350–358.
  
5. Bird AF. Chemical composition of the nematode cuticle;observations on individual layers and extracts from these layers in Ascaris lumbricoides cuticle. Exp Parasitol 1957;6(4):383–403.
  
7. Campbell JW. Nitrogen and amino acid composition of three species of anoplocephalid cestodes: Moniezia expansa, Thysanosoma actinioides, and Cittotaenia perplexa. Exp Parasitol 1960;9:1–8.
  
8. Daugherty JW. Intermediary protein metabolism in helminths. IV. The active absorption of methionine by the cestode, H. diminuta. Exp Parasitol 1957;6(1):60–67.
  
9. Daugherty JW, Foster WB. Comparative studies on amino acid absorption by cestodes. Exp Parasitol 1958;7(1):99–107.
  
10. Foster WB, Daugherty JW. Establishment and distribution of Raillietina cesticillus in the fowl and comparative studies on amino acid metabolism of R. cesticillus and Hymenolepis diminuta. Exp Parasitol 1959;8:413–426.
  
11. Goodchild CG, Wells OC. Amino acids in larval and adult tapeworms (Hymenolepis diminuta) and in the tissues of their rat and beetle hosts. Exp Parasitol 1957;6(6):575–585.
  
12. Hahn SS, et al. The Korean J Intern Med 1961;4:281–285.
13. Hankes LV, Stoner RD. In vitro metabolism of dl-alanine-2-C-14 and glycine-2-C-14 by Trichinella spiralis larvae. Proc Soc Exp Biol Med 1956;91(3):443–446.
 
14. Hankes LV, Stoner RD. Incorporation of DL-tyrosine-2-C-14 and DL-tryptophan-2-C-14 by encysted Trichinella spiralis larvae. Exp Parasitol 1958;7(1):92–98.
  
15. Haskins WT, Weinstein PP. Nitrogenous excretory products of Trichinella spiralis larvae. J Parasitol 1957;43(1):19–24.
  
16. Haskins WT, Weinstein PP. Amino acids excreted by Trichinella spiralis larvae. J Parasitol 1957;43(1):25–27.
  
17. Jaskoski BJ. Paper chromatography of some fractions of Ascaris suum eggs. Exp Parasitol 1962;12:19–24.
  
18. Jennings FW, Mulligan W, Urquhart GM. Radioisotope studies on the anemia produced by infection with Fasciola hepatica. Exp Parasitol 1956;5(5):458–468.
  
19. Johnson AE. The free amino acids in Trichomonas foetus. Exp Parasitol 1962;12:168–175.
  
20. Kent HN. Biochemical studies on the proteins of Hymenolepis diminuta. Exp Parasitol 1957;6(4):351–357.
  
23. Levy AL, et al. Analyt Chem 1953;25:396–399.
 
24. Ma L. J Parasit 1963;49:197–203.
  
25. Prescott DM, Voge M. Autoradiographic study of the synthesis of ribonucleic acid in cysticercoids of Hymenolepis diminuta. J Parasitol 1959;45:587–590.
  
26. Read CP, Simmons JE Jr, Rothman AH. Permeation and membrane transport in animal parasites: amino acid permeation into tapeworms from elasmobranchs. J Parasitol 1960;46:33–41.
  
27. Rogers WP. Amino acids and peptides excreted by nematode parasites. Exp Parasitol 1955;4(1):21–28.
  
28. Rohrbacher GH Jr. Observations on the survival in vitro of bacteria-free adult common liver flukes, Fasciola hepatica Linn., 1758. J Parasitol 1957;43(1):9–18.
  
30. Schroeder WA, Kay LM, Wells IC, GErke C, Moss J. Amino acid composition of hemoglobins of normal Negroes and sickle-cell anemics. J Biol Chem 1950;187(1):221–240.
 
32. Stoner RD, Hankes LV. Incorporation of C14-labeled amino acids by Trichinella spiralis larvae. Exp Parasitol 1955;4(5):435–444.
  
33. Stoner RD, Hankes LV. Not Available Exp Parasitol 1958;7(2):145–151.
 
35. Williams RJ, Kirby H. Paper Chromatography Using Capillary Ascent. Science 1948;107(2784):481–483.
  
36. Williamson J, Desowitz RS. The chemical composition of trypanosomes. I. Protein, amino acid and sugar analysis. Exp Parasitol 1961;11:161–175.
  
37. Iyer GY. Studies on human serum amino acids. I. Separation and identification by paper chromatography. Indian J Med Res 1954;42(2):225–229.
 
38. Yoshizawa T. Jap J Parasit 1954;3:228–232.
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