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Korean J Parasitol > Volume 57(4):2019 > Article
The Korean Journal of Parasitology 2019;57(4):435-437. doi: https://doi.org/10.3347/kjp.2019.57.4.435
Detection of Human Anti-Trypanosoma cruzi Antibody with Recombinant Fragmented Ribosomal P Protein
Yeong Hoon Kim1, Zhaoshou Yang2, Jihoo Lee3, Hye-Jin Ahn2, Chom-Kyu Chong3, Wagner Maricondi4, Ronaldo F. Dias4, Ho-Woo Nam2
1Department of Ophthalmology, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
2Department of Parasitology, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
3GenBody Inc., Cheonan 31116, Korea
4WAMA Diagnostica, Aldo Germano Clein, 100-CEAT, Sao Carlos, CEP. 13573-470, SP-Brazil
* Corresponding Author: Ho-Woo Nam, Email: howoo@catholic.ac.kr
Received: April 15, 2019;  Revised: July 3, 2019;  Accepted: July 15, 2019.
Abstract
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.
Key words: Trypanosoma cruzi, Chagas disease, ribosomal P protein, GST-fusion protein, western blot, patients’ sera
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