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Korean J Parasitol > Volume 35(4):1997 > Article

Brief Communication
Korean J Parasitol. 1997 Dec;35(4):295-298. English.
Published online Dec 20, 1997.  http://dx.doi.org/10.3347/kjp.1997.35.4.295
Copyright © 1997 by The Korean Society for Parasitology
Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type
J R Yu,*1J S Chung,1 and J Y Chai2
1Department of Parasitology, College of Medicine, Kon-Kuk University, Chungju 380-701, Korea.

*Corresponding author (Email: yu@kcucc.konkuk.ac.kr)
Received August 25, 1997; Accepted November 10, 1997.

Abstract

Genomic DNA from Metagonimus yokogawai and Metagonimus Miyata type was amplified by polymerase chain reaction based on the random amplification of polymorphic DNA (RAPD) technique. Eight random 10-mer oligonucleotide primers (OPA-02, 5-TGCCGAGCTG-3; OPA-09, 5-GGGTAACGCC-3; OPA-10, 5-GTGATCGCAG-3; OPA-11, 5-CAATCGCCGT-3; OPA-13, 5-CAGCACCCAC-3; OPA-17, 5-GACCGCTTGT-3; OPA-19, 5-CAAACGTCGG-3; OPA-20, 5-GTTGCGATCC-3) with a G + C content of 60-70% (Kit A, Operon Technologies Inc., California, USA) could produce distinguishable banding patterns between the two Metagonimus species. From the results of this study, it was suggested that Metagonimus Miyata type has a different DNA sequence from M. Yokogawai.

Figures


Fig. 1
Silver stained polyacrylamide gel showing RAPD profiles of Metagonimus species. OPA, primer number; m, Metagonimus Milyata type; y, M. yokogawai; M1, M2, marker.


Fig. 2
Silver stained polyacrylamide gel showing RAPD profiles of Metagonimus species. OPA, primer number; m, Metagonimus miyata type; y, M. yokogawai; M1, M2, marker.

References
1. Chai JY, Huh S, Yu JR, Kook J, Jung KC, Park EC, Sohn WM, Hong ST, Lee SH. An epidemiological study of metagonimiasis along the upper reaches of the Namhan River. Korean J Parasitol 1993;31(2):99–108.
  
2. Chai JY, Sohn WM, Kim MH, Hong ST, Lee SH. Three morphological types of the genus Metagonimus encysted in the dace, Tribolodon taczanowskii, caught from the Sumjin River. Korean J Parasitol 1991;29(3):217–225.
 
3. Kim CH, Kim NM, Lee CH, Park JS. [Studies on the Metagonimus fluke in the Daecheong reservoir and the upper stream of Geum river, Korea]. Korean J Parasitol 1987;25(1):69–82.
 
5. Petrie JL, Burg EF 3rd, Cain GD. Molecular characterization of Echinostoma caproni and E. paraensei by random amplification of polymorphic DNA (RAPD) analysis. J Parasitol 1996;82(2):360–362.
  
6. Saito S. Proceed Japan Parasite Taxon Morphol Meet 1984;2:1–4.
8. Yu JR, Chung JS, Huh S, Lee SH, Chai JY. PCR-RFLP pattern of three kinds of Metagonimus in Korea. Korean J Parasitol 1997;35(4):271–276.
  
9. Yu JR, Kwon SO, Lee SH. Clonorchiasis and metagonimiasis in the inhabitants along Talchongang (River), Chungwon-gun. Korean J Parasitol 1994;32(4):267–269.
  
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