Localization of worm antigen in <i>Neodiplostomum seoulense</i> by immuno-electronmicroscopy

, Lee, J C; , Kong, Y; , Lee, S U; , Huh, S

doi:1997.35.2.95

Korean J Parasito > Volume 35(2):1997 > Article

Original Article


Korean J Parasitol. 1997 Jun;35(2):95-104. English. Published online Jun 20, 1997. http://dx.doi.org/10.3347/kjp.1997.35.2.95
Copyright © 1997 by The Korean Society for Parasitology


Localization of worm antigen in Neodiplostomum seoulense by immuno-electronmicroscopy
J C Lee,¹Y Kong,²S U Lee,³ and S Huh^*³
¹Research and Development Center, Samchundang Pharmatheutical Company, Chunchon 200-060, Korea.

Received February 06, 1997; Accepted March 07, 1997.
Abstract
The localization of worm antigen of Neodiplostomum seoulense was examined by immuno-electronmicroscopic observation. Not only the immunized serum of mice with crude worm extract of N. seoulense but also serum of infected mouse were reacted to the worm section. Using immunized serum as primary antibody, the gold particles were deposited on the rough endoplasmic reticulum of the cell of tribocytic organ, spermatozoa in the seminal vesicle, microvilli of the caecum and vitelline follicle. Using infected serum, gold particles were deposited only on the vitelline follicle prominently. This finding suggested that the tribocytic organ, seminal vesicle, caeca and vitelline follicles may play a role of antigen to immunized serum with crude worm extract of N. seoulense, whereas the vitelline follicle, to the infected serum.

Figures

Figs. 1-2
Fig. 1. Tegument of Neodiplostomum seoulense treated with immunized serum. A few number of gold particles were deposited on the rough endoplasmic reticulum (rER). S: spine, CM: circular muscle, LM: longitudinal muscle, G: granule, M: mitochodria, N: nucleus. Fig. 2. Tegument of N. seoulense treated with infected serum. A very few number of gold particles were deposited on the rER. M: mitochondria, N: nucleus.


	Figs. 3-5 Fig. 3. Tribocytic organ of N. seoulense treated with immunized serum. Gold particles (13 ± 2 particles/0.1 µm²) were deposited on the rough endoplasmic reticulum (ER). G: granule. Fig. 4. Ibid. High magnification. Fig. 5. Tribocytic organ of N. seoulense treated with infected serum. Gold particles (2 ± 1 particles/0.1 µm²) were deposited on the rER. G: granule.

Figs. 6-8
Fig. 6. Spermatozoa in the seminal vesicle treated with immunized serum. Gold particles were deposited on the interstitial matrix between spermatozoa. Fig. 7. Spermatozoa in the seminal vesicle treated with infected serum. Gold particles were deposited on the interstitial matrix between spermatozoa. Fig. 8. Head of spermatozoa in the seminal vesicle treated with immunized serum. Gold particles (31 ± 4 particles/0.1 µm²) were deposited on the space surrounding sperm head.


	Figs. 9-10 Fig. 9. Microvilli of the caeca treated with immunized serum. Gold particles (28 ± 2 particles/0.1 µm²) were deposited on the microvilli (V) of caeca. Fig. 10. Microvilli of the caeca treated with infected serum. Gold particles (4 ± 2 particles/0.1 µm²) were deposited on the microvilli (V) of caeca.

Figs. 11-12
Fig. 11. Vitelline follicle treated with immunized serum. Gold particles (34 ± 4 particles/0.1 µm²) were deposited on the interstitial area between and surrounding the granules of the vitelline follicle (F). Fig. 12. Vitelline follicle treated with infected serum. Gold particles (16 ± 3 particles/0.1 µm²) were deposited on the interstitial area between and surrounding the granules of the vitelline follicle (F).

Tables


	Table 1 Quantitative analysis of immunogold particles in the section of Neodiplostomum seoulense with immunized serum and with infected serum

References


1.	Ahn H, Rim HJ, Kim SJ. [Antigenic localities in the tissues of Metagonimus yokogawai observed by immunogoldlabeling method]. Korean J Parasitol 1991;29(3):245–257.

2.	Chu BD, Rim HJ, Kim SJ. [A study on the body fluid antigen of Clonorchis sinensis using immunogold labeling method]. Korean J Parasitol 1990;28(1):11–23.

3.	Hong ST, Shoop WL. Neodiplostomum seoulense, the emended name for Neodiplostomum seoulensis. Korean J Parasitol 1995;33(4):399.

4.	Huh S, Lee SU, Huh SC. A follow-up examination of intestinal parasitic infections of the Army soldiers in Whachon-gun, Korea. Korean J Parasitol 1994;32(1):61–63.

5.	Huh A, Lee SU, Won MH, Jeong YG, Kwon YH, Choi CS. IgA response in mice infected with Neodiplostomum seoulensis. Korean J Parasitol 1995;33(1):55–60.

7.	Kim HS, Lee OR. [Ultrastructural antigenic localization in Paragonimus iloktsuenensis during developmental stage by immunogold labeling method]. Korean J Parasitol 1995;33(4):365–376.

8.	Kwon OS, Lee JS, Rim HJ, Kim SJ. [Antigenic localities in the tissues of the young adult worm of Paragonimus westermani using immunogold labeling method]. Korean J Parasitol 1991;29(1):31–41.

9.	Rim HJ, Kim SJ, Sun IJ, Lee JS. [Antigenic localities in the tissues of Paragonimus westermani by developmental stages using immunogold labeling method]. Korean J Parasitol 1992;30(1):1–14.

*10.*	Seo BS. Seoul J Med 1990;31:61–96.

*11.*	Yamasaki H, Kominami E, Aoki T. Immunocytochemical localization of a cysteine protease in adult worms of the liver fluke Fasciola sp. Parasitol Res 1992;78(7):574–580.