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Korean J Parasitol > Volume 34(4):1996 > Article

Original Article
Korean J Parasitol. 1996 Dec;34(4):233-238. English.
Published online Dec 20, 1996.  http://dx.doi.org/10.3347/kjp.1996.34.4.233
Copyright © 1996 by The Korean Society for Parasitology
Toxoplasma antibody titers by ELISA and indirect latex agglutination test in pregnant women
Jae-Sook Ryu,1Duk-Young Min,*1Myoung-Hee Ahn,1Han Gyoo Choi,1Sang Chul Rho,2Young Jeon Shin,2Bo youl Choi,2 and Hoo Don Joo3
1Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
2Department of Preventive Medicine, College of Medicine, Hanyang University, Seoul 133-791, Korea.
3National Veterinary Research Institute, RDA, Anyang 430-016, Korea.
Received November 28, 1996; Accepted December 02, 1996.

Abstract

The seroepidemiologic studies on anti-Toxoplasma antibody titers were carried out using ELISA and indirect latex agglutination test. Among 899 sera prepared from pregnant women, 39 cases (4.3%) revealed positive reaction and 218 sera from middle school students showed 4 positive reaction (1.8%) by ELISA. By LAT(newly established by National Veterinary Research Institute, Korea), the sera of 7 pregnant women (0.8%) showed positive reaction. When 80 sera showing ≥1:8 by LAT were used for comparing the results obtained from LAT and Toxotest-MT (Eiken Chemical Co., Japan), 7 cases and 8 sera were positive, respectively. All of 11 sera of proven toxoplasmosis patients showed positive reaction in both tests. Overall proportion of agreement between LAT kit and Toxotest-MT was 0.94(κ-index = 0.632, p<0.01), and LAT was considered to be useful for the screening of toxoplasmosis.

Figures


Fig. 1
Comparison of positive rate between ELISA and LAT.

▪, 899 pregnant women; □, 218 middle school students

In ELISA, cut-off absorbance was 0.25.

In LAT, the positive border line was 1:64.



Fig. 2
Age-specific Toxoplasma antibody positive rate of 39 pregnant women showed positive reaction by ELISA.

Tables


Table 1
Age distribution of pregnant women


Table 2
Comparison of frequencies of ILA titers between LAT and Toxotest-MT


Table 3
Overall proportion of agreement between LAT and Toxotest-MT in 80 pregnant sera with titers over 1:8 by LAT


Table 4
Overall proportion of agreement between IgG-ELISA and LAT in 899 sera of pregnant women

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