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Korean J Parasitol > Volume 34(2):1996 > Article

Brief Communication
Korean J Parasitol. 1996 Jun;34(2):155-157. English.
Published online Jun 20, 1996.  http://dx.doi.org/10.3347/kjp.1996.34.2.155
Copyright © 1996 by The Korean Society for Parasitology
Survival of Cryptosporidium muris (strain MCR) oocysts under cryopreservation
J K Rhee,*1 and B K Park2
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju 561-756, Korea.
Received March 05, 1996; Accepted April 17, 1996.

Abstract

We have successfully maintained Cryptosporidium muris by cryopreservation. Oocysts were suspended in distilled water, stored at - 20℃ for 24 hrs, and then cryopreserved at -70℃. Cryopreserved specimens were slowly thawed at 5℃. Oocysts, which had been cryopreserved for 15 months without cryoprotective agents, retained their infectivity by the mouse titration method. Oocysts stored at 5℃ in 2.5% potassium dichromate failed to retain their infectivity beyond 6.5 months.

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