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Korean J Parasitol > Volume 34(1):1996 > Article

Original Article
Korean J Parasitol. 1996 Mar;34(1):79-85. English.
Published online Mar 20, 1996.  http://dx.doi.org/10.3347/kjp.1996.34.1.79
Copyright © 1996 by The Korean Society for Parasitology
Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil
Dong-Il Chung,*1Hyun-Hee Kong,1Hak-Sun Yu,1Yu-Mi Oh,1Sung-Tae Yee,2 and Young-Jin Lim2
1Department of Parasitology, Kyungpook National University School of Medicine, Taegu 700-422, Korea.
2Department of Parasitology, college of Medicine, Dong-A University, Pusan 602-103, Korea.
Received October 20, 1995; Accepted January 03, 1996.

Abstract

A strain, KA/S2, isolated from Korean soil and morphologically assigned to Acanthamoeba castellanii, was characterized by isoenzyme analysis, and total proteins profile, and mitochondrial (Mt) DNA restriction fragment length polymorphism (RFLP), and compared with four reference strains assigned to the species (the authenitic Castellani, Neff, Ma, and Chang strains). It was found that four isoenzyme, total proteins, and Mt DNA RFLP patterns by eight restriction endonucleases of the strain KA/S2 were identical with those of the Neff strain, isolated from soil of California, USA. The Chang strain was unique in its morphology and total protein patterns. Interstrain polymorphisms of isoenzyme profiles and Mt DNA RFLP patterns were observed among the Castellani, Neff, Ma, and Chang strains. Mt DNA RFLP was confirmed to be highly appropriate for the strain characterization and identification of Acanthamoeba spp.

Figures


Fig. 1
Photomicrographs of the cysts of Acanthamoeba castellanii. A. KA/S2 strain; B. Castellani strain; C. Neff strain; D. Ma strain; E. Chang strain. Bars indicate 10 µm.


Fig. 2
Zymograms for isoenzymes of KA/S2 and reference strains of A. Castellanii separated by polyacrylamide gel isoelectric focusing in pH gradient 3~10. A. Acid phosphatase; B. Leucine amino peptidase; C. Glucose-6-phosphate dehydrogenase; D. Glucose phosphate Isomerase. Numbers above lanes refer to the strains listed in Table 2.


Fig. 3
Total proteins of Acanthamoeba separated by polyacrylamide gel isoelectric focusing in pH gradient 3-7. Numbers above lanes refer to the strains listed in Table 2.


Fig. 4
Agarose gel electrophoretic fingerprints of mitochondrial DNA from KA/S2 and reference strains of A. Castellanii. A.EcoR I digests; B.Hpa I digests; C.Bgl II digests; D.Sca I digests; E.Cla I digests; F.Xba I digests; G.Sst I digests; H.Sal I digests. Numbers above lanes refer to the strains listed in Table 2. Size marker is Hind III digested λ phage DNA (M).

Tables


Table 1
Morphology of Acanthamoeba castellanii KA/S2 and the reference strains


Table 2
Development conditions of enzymes tested (final concentration/100ml)

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