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Korean J Parasitol > Volume 33(4):1995 > Article

Original Article
Korean J Parasitol. 1995 Dec;33(4):349-356. English.
Published online Dec 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.4.349
Copyright © 1995 by The Korean Society for Parasitology
Effect of sodium nitrite on Trichomonas vaginalis
Jae-Sook Ryu,*Joong-Wha Park and Duk-Young Min
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
Received October 21, 1995; Accepted November 13, 1995.

Abstract

We have investigated the action of sodium nitrite on the growth and morphologic changes of T. vaginalis and on the treatment of subcutaneous abscess by trichomonad in mice. Sodium nitrite inhibited the growth of metronidazole-sensitive KT9 isolate and metronidazole- resistant CDC85 strain of T. vaginalis as concentration of 6 mM and 10 mM, respectively. Intraperitoneal injection of sodium nitrite (70 µg, 100 µg, 130 µg/g body weight) did not reduce the size of abscess produced by subcutaneous inoculation of T. vaginalis in mice. T. vaginalis, treated with sodium nitrite at concentration giving about 50% inhibition of growth, showed fissures, many vacuoles and electron-translucent zone in the cytoplasm by transmission electron microscopy. In the case of CDC85 treated with 9 mM sodium nitrite, hydrogenosomal matrical change, destruction of hydrogenosomal membrane, autophagic vacuoles, disappearance of Golgi complex and polysome were notably observed. With above results, it is assumed that sodium nitrite inhibits the growth of metronidazole-sensitive and--resistant strains of T. vaginalis and induces the morphological changes of T. vaginalis although it does not affect in reducing of abscess size by T. vaginalis in mice.

Figures


Figs. 1-4
Fig. 1. A normal T. vaginalis KT9 with a nucleus, hydrogenosomes, Golgi complex and vacuoles are present (×12,000). Fig. 2.T. vaginalis KT9 treated with 4 mM NaNO2. Many vacuoles, fissures and electron-translucent zone (ETZ) are present (×8,000). Fig. 3. Normal T. vaginalis CDC85 (×8,000). Fig. 4. T. vaginalis CDC85 treated with 6 mM NaNO2. Many fissures and vacules are illustrated (×8,000).


Figs. 5-8
T. vaginalis CDC85 treated with 9 mM NaNO2. Fig. 5. Ball-like trophozoite. Autophagic vacuoles and cytoplasmic matrix are widely present (×5,000). Fig. 6. Alteration of electron density of hydrogenosomal matrix and autophagic vacuoles are illustrated (×8,000). Fig. 7. Whirl-like fissure and many vacuoles are observed (×8,000). Fig. 8. Disrupted plasma membrane (arrow) and many autophagic vacuoles are illustrated (×5,000). A, axostyle; AV, autophagic vacuole; ETZ, electron-translucent zone; F, fissure; GC, Golgi complex; GV Golgi vesicle; HG, hydrogenosome; M, cytoplasmic matrix; N, nucleus; RER, rough endoplasmic reticulum; RF, recurrent flagellum; UM, undulating membrane; V, vacuole; VE, vesicle.

Tables


Table 1
Minimal lethal concentration (MLC) of metronidazole for T. vaginalis isolates


Table 2
Effect of sodium nitrite on metronidazole-sensitive KT9 isolate and metronidazole-resistant CDC85 strain of T. vaginalis survival in culture medium


Table 3
Effect of sodium nitrite against subcutaneous infection with metronidazole-sensitive KT9 isolate and metronidazole-resistant CDC86 strain of T. vaginalis in mice

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