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Korean J Parasitol > Volume 31(3):1993 > Article

Original Article
Korean J Parasitol. 1993 Sep;31(3):193-199. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.3.193
Copyright © 1993 by The Korean Society for Parasitology
Detection of Cryptosporidium oocysts from out-patients of the Severance Hospital, Korea
M H Cho,*1A K Kim,1 and K Im2
1Department of Biology, College of Sciences, Kon-Kuk University, Seoul 133-701, Korea.
Received May 14, 1993; Accepted July 06, 1993.

Abstract

A total of 230 randomly collected formalin-fixed fecal samples (submitted to the Severance Hospital, Yonsei University) were selected for tests for human cryptosporidiosis. The stool specimens were examined for Cryptosporidium oocysts by acid-fast (AF) stain, auramin-rhodamine (AR) stain, and monoclonal antibody (mAb) OW3 fluorescence method specific for oocyst wall. Of the 230 stool specimens, 21% were identified by the AF method, 22% were identified by the AR method, and 10% were identified by the mAb fluorescence method, indicating that human Cryptosporidium infections have been existing in Korea.

Figures


Fig. 1
Cryptosporidium oocysts in human stool specimens stained by acid-fast stain technique (A), auramine fluorescent technique (B), and indirect immunofluorescent technique using a commercially available monoclonal antibody (C), respectively. Bar, 5 µm.

Tables


Table 1
Detection of human Cryptosporidium infection by acid-fast staining, auramine-rhodamine staining, and fluorescent mAb-based method

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