| Home | E-Submission | Sitemap | Contact us |  
Korean J Parasitol > Volume 31(2):1993 > Article

Original Article
Korean J Parasitol. 1993 Jun;31(2):83-89. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.2.83
Copyright © 1993 by The Korean Society for Parasitology
Turnover of biliary epithelial cells in Clonorchis sinensis infected rats
S T Hong,1W G Kho,2W H Kim,3J Y Chai,1 and S H Lee*1
1Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received March 26, 1993; Accepted April 13, 1993.


We performed bromodeoxyuridine (BrdU) staining to observe the proliferation pattern of epithelial cells on the biliary mucosa in Clonorchis sinensis infection. Albino rats were infected with 100 metacercariae each and their livers were processed for histopathological observation after BrdU injection. Five to six sites in the liver of a rat were selected for paraffin section, and stained immunohistochemically to visualize BrdU incorporating cells. The flukes were mainly in the common bile duct and right or left hepatic bile ducts. The proportion of stained epithelial cells in the infected bile ducts where the worms were found on the section was 2.9-10.2% at 1 week after infection, 7.3-12.8% at 2 weeks, 7.3-13.4% at 5 weeks, and 8.4-14.8% at 15 weeks while in the non-infected ducts 0 to 2.7% cells were stained. The stained cells were mainly at the base of the mucosal layer. It is suggested that mucosal epithelial cells of the bile ducts infected with C. sinensis become hyperplastic mainly by direct and local stimulation of the worms.


Fig. 1
Distribution of C. sinensis in the schematically illustrated ducts of the rats. The dotted ducts were infected by the worms. The worms were only in the proximal ducts of individual lobes at the first week, and then migrated further into the distal ducts at the second week. At 5 or 15 weeks after infection the worms were found in the proximal ducts but not in the distal ducts. The right lateral lobe (R) was not examined. CBD: common bile duct, CP: caudate lobe proximal duct, CD: caudate lobe distal duct, LP: left lateral lobe proximal duct, LD: left lateral lobe distal duct, MP: median lobe proximal duct, MD: median lobe distal ducts, R: right lateral lobe (excluded in this examination).

Figs. 2-3
Fig. 2. A proximal intrahepatic bile duct in the median lobe was narrow and lined by monolayer of epithelial cells. No cells were incorporating BrdU. Original magnification, × 200. Fig. 3. An infected proximal intrahepatic bile duct in the median lobe by C. sinensis was markedly dilated and showed severe hyperplasia of epithelial cells 2 weeks after infection. Numerous cells(cells with brown nuclei) were incorporating BrdU at the base of the hyperplastic epithelial layer. Original magnification, × 200.


Table 1
Proportion (%) of BrdU incorporating cells on the biliary mucosa and location of the worms in the liver of rats infected by C. sinensis

Table 2
Proportion (%) of BrdU incorporating cells on the biliary mucosa in the liver of rats infected by C. sinensis

2. Chung CS, et al. Korean J Pathol 1976;10(1):33–46.
3. Flavell DJ. Liver-fluke infection as an aetiological factor in bile-duct carcinoma of man. Trans R Soc Trop Med Hyg 1981;75(6):814–824.
4. Hahm JH, et al. Korea Univ Med J 1984;21:177–184.
5. Hong ST, et al. Seoul J Med 1990;31(2):117–127.
6. Kim SS, Han MH, Park SG, Lim HS, Hong ST. [A survey on the epidemiological factors of clonorchiasis in the Pohang industrial belt along the Hyungsan river, Kyongsangbuk-do]. Korean J Parasitol 1990;28(4):213–219.
7. Kim SS, et al. Inje Med J 1984;5(4):379–385.
8. Kim YI. Liver carcinoma and liver fluke infection. Arzneimittelforschung 1984;34(9B):1121–1126.
9. Lee JH, Rim HJ, Bak UB. Effect of Clonorchis sinensis infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian golden hamsters. Korean J Parasitol 1993;31(1):21–30.
10. Lee SH, et al. Seoul J Med 1988;29(3):253–262.
11. Lee SH, Hong ST, Kim CS, Sohn WM, Chai JY, Lee YS. Histopathological changes of the liver after praziquantel treatment in Clonorchis sinensis infected rabbits. Korean J Parasitol 1987;25(2):110–122.
12. Lee SH, Shim TS, Lee SM, Chi JG. [Studies On Pathological Changes Of The Liver In Abino Rats Infected With Clonorchis Sinensis]. Korean J Parasitol 1978;16(2):148–155.
13. Min DY, Ahn MH, Kim KM, Kim CW. [Intestinal parasite survey in Seoul by stool examination at Hanyang University Hospital]. Korean J Parasitol 1986;24(2):209–212.
14. Rim HJ. The current pathobiology and chemotherapy of clonorchiasis. Korean J Parasitol 1986;24 Suppl:1–141.
15. Risio M, Coverlizza S, Ferrari A, Candelaresi GL, Rossini FP. Immunohistochemical study of epithelial cell proliferation in hyperplastic polyps, adenomas, and adenocarcinomas of the large bowel. Gastroenterology 1988;94(4):899–906.
16. Song GA, et al. Korean J Int Med 1989;37(3):344–355.
17. Tarao K, Shimizu A, Harada M, Ohkawa S, Okamoto N, Kuni Y, Ito Y, Tamai S, Iimori K, Sugimasa I, et al. In vitro uptake of bromodeoxyuridine by human hepatocellular carcinoma and its relation to histopathologic findings and biologic behavior. Cancer 1991;68(8):1789–1794.
Editorial Office
c/o Department of Medical Environmental Biology
Chung-AngUniversity College of Medicine, Dongjak-gu, Seoul 06974, Korea
Tel: +82-2-820-5683   Fax: +82-2-826-1123   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2020 by The Korean Society for Parasitology and Tropical Medicine. All rights reserved.     powerd by m2community