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Korean J Parasitol > Volume 31(2):1993 > Article

Original Article
Korean J Parasitol. 1993 Jun;31(2):83-89. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.2.83
Copyright © 1993 by The Korean Society for Parasitology
Turnover of biliary epithelial cells in Clonorchis sinensis infected rats
S T Hong,1W G Kho,2W H Kim,3J Y Chai,1 and S H Lee*1
1Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received March 26, 1993; Accepted April 13, 1993.

Abstract

We performed bromodeoxyuridine (BrdU) staining to observe the proliferation pattern of epithelial cells on the biliary mucosa in Clonorchis sinensis infection. Albino rats were infected with 100 metacercariae each and their livers were processed for histopathological observation after BrdU injection. Five to six sites in the liver of a rat were selected for paraffin section, and stained immunohistochemically to visualize BrdU incorporating cells. The flukes were mainly in the common bile duct and right or left hepatic bile ducts. The proportion of stained epithelial cells in the infected bile ducts where the worms were found on the section was 2.9-10.2% at 1 week after infection, 7.3-12.8% at 2 weeks, 7.3-13.4% at 5 weeks, and 8.4-14.8% at 15 weeks while in the non-infected ducts 0 to 2.7% cells were stained. The stained cells were mainly at the base of the mucosal layer. It is suggested that mucosal epithelial cells of the bile ducts infected with C. sinensis become hyperplastic mainly by direct and local stimulation of the worms.

Figures


Fig. 1
Distribution of C. sinensis in the schematically illustrated ducts of the rats. The dotted ducts were infected by the worms. The worms were only in the proximal ducts of individual lobes at the first week, and then migrated further into the distal ducts at the second week. At 5 or 15 weeks after infection the worms were found in the proximal ducts but not in the distal ducts. The right lateral lobe (R) was not examined. CBD: common bile duct, CP: caudate lobe proximal duct, CD: caudate lobe distal duct, LP: left lateral lobe proximal duct, LD: left lateral lobe distal duct, MP: median lobe proximal duct, MD: median lobe distal ducts, R: right lateral lobe (excluded in this examination).


Figs. 2-3
Fig. 2. A proximal intrahepatic bile duct in the median lobe was narrow and lined by monolayer of epithelial cells. No cells were incorporating BrdU. Original magnification, × 200. Fig. 3. An infected proximal intrahepatic bile duct in the median lobe by C. sinensis was markedly dilated and showed severe hyperplasia of epithelial cells 2 weeks after infection. Numerous cells(cells with brown nuclei) were incorporating BrdU at the base of the hyperplastic epithelial layer. Original magnification, × 200.

Tables


Table 1
Proportion (%) of BrdU incorporating cells on the biliary mucosa and location of the worms in the liver of rats infected by C. sinensis


Table 2
Proportion (%) of BrdU incorporating cells on the biliary mucosa in the liver of rats infected by C. sinensis

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