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Korean J Parasitol > Volume 31(1):1993 > Article

Original Article
Korean J Parasitol. 1993 Mar;31(1):49-56. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.1.49
Copyright © 1993 by The Korean Society for Parasitology
Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis
Jong Hyun Lee,Yoon Kong,Jae-Young Ryu and Seung-Yull Cho*
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Received December 19, 1992; Accepted January 26, 1993.

Abstract

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has widely been applied. This study was undertaken to observe whether diagnostic capability can be improved by applying more sensitive techniques such as Protein A-ELISA and avidin biotin complex ELISA (ABC-ELISA). When evaluated using 115 sera of human cysticercosis, the antibody positive rates were not significantly improved in Protein A-ELISA (82.6%) and in ABC-ELISA (86.1%) than in micro-ELISA (81.7%). The specificities, evaluated in 165 sera from other diseases and normal controls, were significantly improved (88.5% by micro-ELISA, 93.3% by Protein A-ELISA and 93.8% by ABC-ELISA). Antibody levels (absorbance, abs.) in individual serum were correlated well (r = 0.83-0.86) each other. An actual benefit of Protein A-ELISA and ABC-ELISA was that they needed smaller amount of test sample.

Figures


Fig. 1
Relations of the individual antibody levels (abs.) as measured by micro-ELISA and Protein A-ELISA in sera of 115 cysticercosis patients. Horizontal and vertical dotted lines of abs. 0.18 or 0.10 are the positive criteria.


Fig. 2
Relations of the individual antibody levels (abs.) as measured by micro-ELISA and ABC-ELISA in sera of 115 cysticercosis patients. Dotted lines at abs. 0.18 and 0.10 are the same as described in Fig. 1.


Fig. 3
Distribution and relation of the specific antibody levels (abs.) between micro-ELISA and Protein A-ELISA to T. solium metacestode antigen in 165 sera of other diseases and normal controls. Horizontal and vertical lines of abs. 0.18 and 0.10 are the positive criteria.


Fig. 4
Distribution and relation of the specific antibody levels (abs.) between micro-ELISA and ABC-ELISA to T. solium metacestode antigen in 165 sera of other diseases and normal controls. Lines and symbols are the same as described in Fig. 3.

Tables


Table 1
Procedures and conditions of 3 different ELISA techniques employed in this study


Table 2
Results of specific-antibody tests by 3 different techniques of ELISA in each category of patients


Table 3
Sensitivity and specificity of three techqniques of ELISA as calculated in 115 cysticercosis and 165 other diseases and normal control

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