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Korean J Parasitol > Volume 31(1):1993 > Article

Original Article
Korean J Parasitol. 1993 Mar;31(1):43-48. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.1.43
Copyright © 1993 by The Korean Society for Parasitology
Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera
Seoung Cheol Lee,Young-Bae Chung,Yoon Kong,Shin-Yong Kang and Seung-Yull Cho*
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Received December 19, 1992; Accepted January 18, 1993.

Abstract

Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by reacting human sera from clinically diagnosed metagonimiasis, fascioliasis, clonorchiasis and paragonimiasis with 4 crude extracts of Metagonimus yokogawai (metacercariae), adults of Fasciola hepatica, Clonorchis sinensis and Paragonimus westermani. By ELISA, 10 of 11 metagonimiasis sera showed the highest absorbance (abs.) to the homologous antigen. Cross reactions to M. yokogawai antigen occurred most frequently in clonorchiasis sera. The antigenic protein fractions in M. yokogawai metacercarial extract were observed by SDS-PAGE/immunoblot using patients and control sera together with experimental cat sera. Out of 14 protein bands in the extract, 11 bands were reacting. Cross reacting bands to other trematodiasis sera were frequently observed. Of the reacting bands, 66 and 22 kDa proteins were recognized as specific for metagonimiasis.

Figures


Fig. 1
SDS-PAGE findings of crude saline extract of M. yokogawai metacercariae. 7.5-15% resolving gel was used for protein separation. Mr: Molecular mass markers, Mc: Crude extract of metacercariae.


Fig. 2
SDS-PAGE/immunoblot findings of crude saline extract of M. yokogawai metacercariae with trematodiases sera. A: Experimental cat, B: Clinical patients of metagonimiasis. C: Clonorchiasis, D: Fascioliasis, E: Paragonimiasis, F: Normal control. Numerical at the left stand for the molecular mass (kDa). Open triangles (◃) show the specific bands for metagonimiasis. Numbers at the top of each lane indicate case number.

Tables


Table 1
Mean and standard deviation of antibody levels (abs.) as measured by micro-ELISA in patients sera of trematodiases. Protein concentrations of 2.5 µg/ml was standardized in each trematode except for Clonorchis which coated at 5 µg/ml of protein

References
1. Chai JY, et al. Seoul J Med 1989;30:139–142.
2. Chi JG, Kim CW, Kim JR, Hong ST, Lee SH. Intestinal pathology in human metagonimiasis with ultrastructural observations of parasites. J Korean Med Sci 1988;3(4):171–177.
 
3. Cho SY, Kang SY, Lee JB. Metagonimiasis in Korea. Arzneimittelforschung 1984;34(9B):1211–1213.
 
4. Cho SY, Kim SI, Kang SY. Specific IgG antibody responses in experimental cat metagonimiasis. Korean J Parasitol 1987;25(2):149–153.
 
5. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.
  
6. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
 
7. McLaren M, Draper CC, Roberts JM, Minter-Goedbloed E, Ligthart GS, Teesdale CH, Amin MA, Omer AH, Bartlett A, Voller A. Studies on the enzyme linked immunosorbent assay (ELISA) test for Schistosoma mansoni infections. Ann Trop Med Parasitol 1978;72(3):243–253.
 
8. Seo BS, Lee SH, Cho SY, Chai JY, Hong ST, Han IS, Sohn JS, Cho BH, Ahn SR, Lee SK, Chung SC, Kang KS, Shim HS, Hwang IS. An Epidemiologic Study On Clonorchiasis And Metagonimiasis In Riverside Areas In Korea. Korean J Parasitol 1981;19(2):137–150.
 
9. Tsang VC, Peralta JM, Simons AR. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol 1983;92:377–391.
  
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