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Korean J Parasitol > Volume 31(1):1993 > Article

Original Article
Korean J Parasitol. 1993 Mar;31(1):21-30. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.1.21
Copyright © 1993 by The Korean Society for Parasitology
Effect of Clonorchis sinensis infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian golden hamsters
Jae-Hyun Lee,1Han-Jong Rim,*1 and Ung-Bok Bak2
1Department of Parasitology and Institute for Tropical Endemic Diseases, College of Medicine, Korea University, Seoul 136-701, Korea.
2Department of Pathology, College of veterinary Medicine, Seoul National University, Suwon 441-744, Korea.
Received December 30, 1992; Accepted February 04, 1993.


The study was carried out to observe the effects of Clonorchis sinensis infection on induction of cholangiocarcinoma in Syrian golden hamsters to which 15 ppm dimethylnitrosamine (DMN) solution was administered for 8 weeks. The histopathological changes of the bile duct and liver cells were observed at the 11th week. In six of 8 hamsters (75%) which were treated with DMN and then infected with C. sinensis, the livers developed cholangiocarcinoma at 10 weeks after the infestation of C. sinensis. The features of cholangiocarcinoma lesions were adenomatous or papillary hyperplasia of the bile duct epithelia showing distinct anaplastic changes with mucinous cell metaplasia and necrotic area. In the hamsters which received either DMN or C. sinensis alone, the livers showed only hyperplastic changes of the bile duct epithelial cells. It was suggested that C. sinensis infection and DMN administration could be a synergism on the development of cholangiocarcinoma in Syrian golden hamsters.


Fig. 1
Changes of body weights in the hamsters. ↓ : P < 0.001, each group is significantly different from control group.

Fig. 2
Average body weights and percent of liver weight to body weight in each group at the 11th week. ↓ : Percentages of gr. I and gr. II to gr. IV are significantly high (P < 0.001).

Figs. 3-6
Fig. 3. Cut surface of the liver of a hamster treated with DMN and C. sinensis shows gray-white masses (arrow heads) 0.2-0.5 cm in diameter. Fig. 4. Microscopic appearance of the liver of a hamster infected with C. sinensis shows the dilated bile duct containing the worm (C) and the hyperplasia of bile duct epithelia (arrow). Hematoxylin and eosin (HE) stains (× 100) Fig. 5. Microscopic appearance of the liver of a hamster treated with DMN shows adenomatous hyperplasia of bile duct epithelia. HE stains (× 200) Fig. 6. Microscopic appearance of the liver of a hamster treated with DMN and C. sinensis shows papillary proliferation of the epithelium. HE stains (× 100)

Figs. 7-8
Fig. 7. In high magnification of Fig. 6, the bile duct shows carcinomatous changes such as irregular stratification, pleomorphism and mitosis (arrow heads) of epithelial cells. HE (× 400) Fig. 8. Microscopic appearance of cholangiocarcinoma shows a lot of metaplastic goblet cells (arrows) in DMN plus C. sinensis group. Alcian blue and periodic acid Schiff stains (AB-PAS) (× 200)


Table 1
Gross lesions of the liver of hamsters

Table 2
Occurrence numbers for histological lesions of bile duct of hamsters in each group

Table 3
Occurrence numbers of other histopathological changes of the livers in each group

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