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Korean J Parasitol > Volume 31(1):1993 > Article

Original Article
Korean J Parasitol. 1993 Mar;31(1):13-20. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.1.13
Copyright © 1993 by The Korean Society for Parasitology
Secretions of the biliary mucosa in experimental clonorchiasis
Soon-Hyung Lee,*Jong-Inn Lee,1Sun Huh,2Jae-Ran Yu,3Sei Woo Chung,4Jong-Yil Chai and Sung-Tae Hong
Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.

* Present Address: 1National Cancer Hospital, Dept. of General surgery, Seoul 139-240; 2Hallym University, Dept. of Parasitology, Chunchon 200-702; 3Kon-kuk University, Dept. of Parasitology, Chungju 380-701; 4Dept. of General surgery, College of Medicine, Inha University, Inchon 402-751.

Received November 02, 1992; Accepted January 07, 1993.


The histological change of the biliary mucosa in clonorchiasis is characterized as adenomatous hyperplasia, and cross-sectioned mucosa looks like intestinal mucosa. In addition to the glandular hyperplasia, the metaplasia of mucin secreting cells is also known. The present study investigated the presence of intestinal secretion from the biliary mucosal cells of rabbits and rats with Clonorchis sinensis infection. The rabbit was infected with 300 and the rat was infected with 100 metacercariae of C. sinensis. A part of the animals were followed up after praziquantel treatment. The rabbit livers were prepared for histochemistry to observe any endocrine secretion and the bile duct mucosa of the mice was processed for the activity of brush border membrane (BBM)-bound enzymes of the small intestine. Immunohistochemistry with the polyclonal antibodies and biotin-streptavidin-peroxidase staining kit showed no positive cells for gastrin and secretin, but a few cells were positive for serotonin. The proliferated biliary mucosa of the mice revealed no activity of disaccharidases and aminopeptidase. Only alkaline phosphatase activity was found both in the control and the infected. The hyperplastic biliary mucosal cells showed no gastrointestinal secretory functions. The serotonin secreting cells may be one of the inflammatory cells.


Figs. 1-4
Fig. 1. Mucosa of the stomach as a positive staining for gastrin, × 200. Fig. 2. The proximal intrahepatic bile duct of an infected rabbit with negative gastrin staining, × 100. Fig. 3. Mucosa of the jejunum as a positive staining for secretin, × 400. Fig. 4. Negative secretin staining in the biliary mucosa of an infected rabbit, × 200.

Figs. 5-12
Fig. 5. Serotonin positive cells in the jejunum as a positive reference staining, × 400. Figs. 6-10. Serotonin positive cells (arrows) in the biliary mucosa of the infected rabbits, × 400. Fig. 11. Negative serotonin staining in the biliary mucosa of the infected rabbits 6 months after treatment, × 200. Fig. 12. Negative serotonin staining in the biliary mucosa of the infected rabbits 12 months after treatment, × 100.


Table 1
Immunohistochemical findings of the biliary mucosa in rabbit clonorchiais

Table 2
Activities of the brush border membrane bound enzymes in the biliary mucosa of rats infected with C. sinensis (unit: mu/mg protein)

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