Comparative antigen analysis of <i>Trichomonas vaginalis</i> by enzyme-linked immunoelectrotransfer blot technique

, Min, D Y; , Leem, M H; , Kim, J M; , Choi, Y K

doi:1992.30.4.323

Korean J Parasito > Volume 30(4):1992 > Article

Original Article


Korean J Parasitol. 1992 Dec;30(4):323-328. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1992.30.4.323
Copyright © 1992 by The Korean Society for Parasitology


Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique
D Y Min,M H Leem,J M Kim and Y K Choi^*
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.


Abstract
Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed 25 protein profiles ranging 12-170 kDa of molecular weight in SDS-PAGE. In EITB, the specific immunogenic bands were visualized at 51 kDa and 96 kDa when HY-1 antigen was probed with different mice sera immunized with 6 isolates of T. vaginalis. The banding patterns with different sera showed isolate-to-isolate variability. In EITB, homologous antigen (HY-1) did not show any enhanced response in reacting to homologous antiserum (HY-1) when 6 isolates of T. vaginalis were probed with a single serum (HY-1). It is assumed that the different banding patterns of six isolates show isolate-to-isolate variability and immunogenic common bands in 41, 47, 74 and 94 kDa on EITB may connote the important significance on immune response in T. vaginalis infection.

Figures


	Fig. 1 DSD-PAGE of TCA-treated(lane 1), sonicated(lane 2) and detergent-treated(lane 3) proteins of pathogenic human T. vaginalis isolate HY-1.


	Fig. 2 SDS-PAGE of six pathogenic human T. vaginalis isolates treated with TCA. Lane 3; HY-1, lane 4; HY-3, lane 5; HY-13, lane 6; HY-2, lane 7; HY-9, lane 8; HY-10.


	Fig. 3 Immunoblot of a singlc isolatc(HY-1) of T. vaginalis probed with normal mouse serum(lane 1) or serum from mice immunized with isolates HY-1(lane 2), HY-2(lane 3), HY-3(lane 4), HY-9(lane 5), HY-10(lane 6) and HY-13(lane 7).

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