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Changes in blastogenic responses and antibody titers of mice infected with Toxoplasma gondii
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Korean J Parasito > Volume 30(2):1992 > Article

Original Article
Korean J Parasitol. 1992 Jun;30(2):125-131. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.2.125
Copyright © 1992 by The Korean Society for Parasitology
Changes in blastogenic responses and antibody titers of mice infected with Toxoplasma gondii
D W Shin,Y H Lee,Y E Na and D S Kwon
Department of Parasitology, College of Medicine, Chungnam National University, Daejeon 301-131, Korea.
Abstract

This study was performed to observe the cell-mediated and humoral immune responses in mice which were infected with Beverley, Fukaya and ME49 strain of Toxoplasma gondii, respectively. The blastogenic responses of splenocytes using [3H]-thymidine and serum antibody titers were measured weekly up to 10 weeks after infection. The blastogenic responses of splenocytes treated with concanavalin A and Toxoplasma lysate were significantly declined in the 3 strain groups as compared with the non-infected group (p < 0.05), however lipopolysaccharide-treated blastogenic responses were not significantly different between infected and non-infected groups. The serum IgG antibody titers in the three infected groups increased from 2 weeks after infection, and the serum IgM antibody titers increased until 4 weeks after infection. No significant differences were revealed in blastogenic responses and serum antibody titers among the 3 groups. The present study suggested that cell-mediated immune responses were involved in T. gondii infected mice and blastogenic responses of T lymphocytes were inhibited in acute T. gondii infection.

Figures


Fig. 1
Stimulation index of concanavalin A-treated splenocytes from Toxoplasma gondii infected and control mice(mean).

*Stimulation index = (counts per of stimulated splenocytes)/(counts per minute of unstimulated splenocytes)



Fig. 2
Stimulation index of lipopolysaccharide-treated splenocytes from Toxoplasma gondii infected and control mice(mean).

*Stimulation index: refer to Fig. 1.



Fig. 3
Stimulation index of Toxoplasma lysate-treated splenocytes from Toxoplasma gondii infected and control mice(mean).

*Stimulation index: refer to Fig. 1.



Fig. 4
Serum IgG antibody titers as measured by ELISA in Toxoplasma gondii infected and control mice(mean).

ELISA*: enzyme-linked immunosorbent assay



Fig. 5
Serum IgM antibody titers as measured by ELISA in Toxoplasma gondii infected and control mice(mean).

ELISA*: refer to Fig. 4.


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