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Blastogenesis of splenic lymphocytes to specific antigens and PHA in Paragonimus westermani infected mice
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Korean J Parasito > Volume 30(1):1992 > Article

Original Article
Korean J Parasitol. 1992 Mar;30(1):43-48. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.1.43
Copyright © 1992 by The Korean Society for Parasitology
Blastogenesis of splenic lymphocytes to specific antigens and PHA in Paragonimus westermani infected mice
D Y Min,M H Shin and R Choi
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
Abstract

Paragonimus westermani is a common fluke in Korea. The present study aimed to observe the cell mediated immune response in experimental paragonimiasis of mice. The mouse (BALB/c) was orally inoculated with 40 metacercariae of P. westermani from Cambaroides similis. During the infection (1, 2, 4, 6 weeks) of mouse, blastogenic response of splenic lymphocytes to P. westermani adult antigen, metacercaria antigen, and PHA were observed. Sera from infected and noninfected mice added to normal mouse splenic lymphocytes with or without PHA. The blastogenic response of splenic lymphocytes to PHA was reduced after 1 week of infection. However after 6 weeks of infection, the response was restored to the control level. The blastogenic response of splenic lymphocytes to P. westermani adult or metacercaria antigen increased significantly on 1 week after infection, and maintained up to 6 weeks after infection. The response of non-infected mice was suppressed by addition of the infected mouse serum. The present results suggested that cellular immunity was involved in P. westermani infected mice and that P. westermani anti-serum inhibited proliferation of T lymphocytes.

Figures


Fig. 1
Enhancement rate of mouse splenocytes after treatment with mitogens (PHA: phytohemagglutinin, MA: metacercarial antigen, AWA: adult worm antigen).

Tables


Table 1
Stimulation index of mouse splenic lymphocytes after treatment with mitogens


Table 2
Suppression rate of noninfected mouse splenic lymphocytes after addition of infected mouse sera

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