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Korean J Parasitol > Volume 29(4):1991 > Article

Original Article
Korean J Parasitol. 1991 Dec;29(4):397-401. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.4.397
Copyright © 1991 by The Korean Society for Parasitology
Serum IgE levels in rats infected with Paragonimus westermani
M H Shin,J S Ryu and D Y Min
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.

Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was inoculated orally with 20-25 metacercariae of P. westermani from Cambaroides similis. Before and after infection (1, 2, 3, 4, 6, 8 weeks) of P. westermani, the blood was collected from the retro-orbital venous plexus of rats and kept serum at -70℃. Serum total IgE and specific IgG levels were determined by the capture and conventional enzyme-linked immunosorbent assay, respectively.

The results were as follows;

1. Serum IgE values were increased to 0.18 ± 0.042 at 2 weeks, 0.28 ± 0.151 at 4 weeks and 0.43 ± 0.055 at 8 weeks after infection. The absorbances of non-infected rats ranged 0.07 ± 0.021-0.12 ± 0.025.

2. Specific IgG values were slightly increased at 3 weeks (0.20 ± 0.032) and gradually increased up to 8 weeks (0.31 ± 0.067) after infection. The absorbances of non-infected rats ranged 0.11 ± 0.035-0.18 ± 0.019. The present results suggested that P. westermani could elevate serum IgE and specific IgG antibodies in Wistar rats which were not a good definitive host.


Table 1
Serum IgE values in rats infected with P. westermani

Table 2
Serum IgG values in rats infected with P. westermani

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