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Cytotoxicity of lymphokine activated peritoneal macrophages against Trichomonas vaginalis
K Yoon,J S Ryu and D Y Min
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
Abstract
Trichomonas vaginalis is a parasitic flagellate in the urogenital tract of human. Innate cytotoxicity of macrophages against T. vaginalis has been recognized, but any report on the cytotoxicity of lymphokine-activated macrophages to T. vaginalis is not yet available. The present study aimed to elucidate the lymphokine-activated cell mediated cytotoxic effect against T. vaginalis by mouse peritoneal macrophages. Cytotoxicity was measured by counting the release of 3H-thymidine from prelabeled protozoa, and tested in U-bottom microtiter plates. Nitrite concentration in culture supernatants was measured by standard Griess reaction.
The results obtained are as follows:
1. The cytotoxicity of macrophages was increased by addition of rIL-2 or rIFN-γ.
2. Cytotoxicity of macrophages was reduced by addition of rIL-4 to rGM-CSF, rIL-2 or rIFN-γ.
3. Crude lymphokine mixed with anti-IL-2 decreased the cytotoxicity of macrophages.
4. In case of macrophages cultured with rIFN-γ or rIL-4, the concentration of nitrite was related with cytotoxicity of macrophages against T. vaginalis, but the cytotoxicity of macrophages cultured with rIL-2 and rIFN-γ was decreased in spite of its high production of nitrite.
From the results obtained, it is assumed that rIL-2 and rIFN-γ enhance the cytotoxicity of macrophages while rIL-4 inhibits the cytotoxicity against T. vaginalis, and that the production of nitrite does not relate with the cytotoxicity of macrophages, but nitric oxide may play a role as an inhibitory factor on the proliferation of T. vaginalis.
Figures
Fig. 1 Cytotoxicity of macrophages activated by rGM-CSF against T. vaginalis.
Fig. 2 Cytotoxicity of macrophages activated by rIL-2 against T. vaginalis.
Fig. 3 Cytotoxicity of macrophages activated by rIFN-γ against T. vaginalis.
Fig. 4 Cytotoxicity of macrophages activated by rIL-4 against T. vaginalis.
Tables
Table 1 Cytotoxicity of macrophages activated by various concentration of recombinant lymphokine against T. vaginalis
Table 2 Cytotoxicity of recombinant lymphokine-activated macrophages against T. vaginalis
Table 3 Cytotoxicity of macrophages activated by combined recombinant lymphokine against T. vaginalis
Table 4 Effect of lymphokine activated macrophages on the proliferation of T. vaginalis
Table 5 Effect of various concentration of crude lymphokine and anti-lymphokine antibody on the cytotoxicity of macrophages against T. vaginalis
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