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Korean J Parasitol > Volume 28(3):1990 > Article

Original Article
Korean J Parasitol. 1990 Sep;28(3):161-173. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.3.161
Copyright © 1990 by The Korean Society for Parasitology
Experimental life history of Spirometra erinacei
S H Lee,J S We,W M Sohn,*S T Hong and J Y Chai
Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-460, Korea.

**Present address: Department of Parasitology, College of Medicine, Inje University, Pusan 614-735, Korea.

Abstract

The complete life cycle of Spirometra erinacei has been experimentally maintained in the laboratory. The cyclops were reared as the first intermediate host, and the tadpoles of Rana nigromaculata as the second intermediate host. ICR mice were used as another second host. The experimental definitive hosts were dogs and cats. Maturation and hatching of the eggs took 8 to 14 days by incubation at 29℃. The coracidium measured 43.8 × 36.9 µm. Mesocyclops leuckarti and Eucyclops serrulatus were susceptible to the coracidial infection. The procercoids older than 5 days in the cyclops had minute spines at the anterior end, calcium corpuscles in the body parenchyme and the cercomer at the posterior end. Procercoids 10 to 20 days old were infective to tadpoles, and 15 or 21 day old worms could infect the mice. The plerocercoids from the tadpoles at 15 days after experimental infection were pear-shaped and shorter than 1 mm in the length and were infective to mice. Fifteen to 18 days after experimental inoculation of plerocercoids to dogs or cats, the adult worms began to produce eggs. One life cycle from egg to egg needed 48 to 67 days in the laboratory. The morphology of larval or adult worms was compatible with the description of Spirometra erinacei.

Figures


Figs. 1-6
Figs. 1~5. Development of the embryo.

1. The egg of Spirometra erinacei from an experimentally indected dog.

2. The egg after 3 days of incubation.

3. The egg after 5 days of incubation.

4. The egg after 8 days of incubation.

5. The egg after 13 days of development, contained fully mature embryo, showing hoolets (arrow heads).

Fig. 6. The egg which already hatched out.

*Bar unit is µm.



Figs. 7-11
Fig. 7. Coracidium of S. erinacei showing numerous cilia and hooklets(arrow heads).

Fgis. 8~11. Procercoids of S. erinacei in the body cavity of experimental cyclops.

8. A 1-day old one.

9. A 3-day old one.

10. A 5-day old one, showing minute epines at the anterior end, calcium corpuscles(arrows), and cercomer(C).

11. 11-day old ones.

*All bar unit is µm



Figs. 12-15
Fig. 12. 15-day old procercoids in the body cavity of experimental cyclops.

Fig. 13. Magnification of Fig. 12.

Fig. 14. A 17-day old procercoid.

Fig. 15. 30-day old procercoids, calcified in cyclops.

*All bar unit is µm



Figs. 16-19
Figs. 16~19. Plerocercoids from the experimental tadpoles and mice.

16. Two plerocercoids in the mesentery of a tadpole.

17. A 20-day old one.

18. Two 45-day old ones.

19. Three from the experimental mouse.

*All bar and scale unit is mm.



Figs. 20-23
Fig. 20. Whole strobila of S. erinacei from an sxperimental dog at 6 weeks after infection.

Fig. 21. Scolex of S. erinacei.

Fig. 22. Cross section of the scolex.

Fig. 23. Mature proglottids of S. erinacei.



Figs. 24-26
Fig. 24. Genital organs in mature proglottid, showing genital pore(GP), vaginal pore(VP), uterine pore(UP), uterine coil(UC), and ovary(○).

Fig. 25. A longitudinal section of mature proglottids, showing the relationships of genital organ: seminal vesicle(SV), cirrus sac(CS), genital pore(*), vaginal pore(▲), uterus(U), uterine pore(•) and ovary(◦).

Fig. 26. A transverse section of a mature proglottid, showing longitudinal muscle(LM), transverse muscle(TM), testis(T) and vitelline follicles(VF).

*The bar unit is mm


Tables


Table 1
Measurenments of S. erinacei coracidia*


Table 2
Measurenments of S. erinacei procercoids* according to the age of infection


Table 3
The infectivity of S. erinacei procercoids to tadpoles


Table 4
Measurements of spargana* collected from experimental tadpoles


Table 5
The infectivity of S. erinacei proceroids to mice


Table 6
The infectivity of S. erinacei plerocercoids to mice


Table 7
The periods for development of S. erinacei by stages


Table 8
Measurements* of S. erinacei mature proglottids from the experimentally infected dog

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