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Korean J Parasitol > Volume 28(2):1990 > Article

Original Article
Korean J Parasitol. 1990 Jun;28(2):91-100. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.2.91
Copyright © 1990 by The Korean Society for Parasitology
The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani
D Y Min,M H Ahn,K M Kim,M H Leem and S Y Park
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.
Abstract

Paragonimus westermani is a tissue migrating parasite in the early stage until arriving at lung, and most of the parasites spend their life spans there. Considerable immune responses including activation of macrophages are taken place during the residence of parasites in the host. However, concerning the immunologic defense mechanisms of the host against this parasite, only a few document is available so far. In this study, the cytotoxic effect of peritoneal macrophages under the presence of antibody and/or complement against metacercariae of P. westermani was investigated in vitro.

Metacercariae were collected from the crayfish, Cambaroides similis and hatched out in Tyrode solution (pH 7.4). Plastic adherent cells from normal or infected rat (Wistar) peritoneal exudates were used as experimental macrophages. Polyclonal antibodies were obtained from infected rats and a cat. Cat IgG was fractioned with ion exchange chromatography. Fresh rabbit complement was used according to experimental scheme. Various combinations of peritoneal macrophages, normal or infected rat serum, complement and cat IgG were incubated at 36℃ in 5% CO2 incubator for 6, 14, 24 and 48 hours.

The results obtained were as follows:

1. P. westermani infection activated peritoneal macrophages non-specifically and this activation induced increases of cell adherence and cytotoxicity on metacercariae.

2. In the presence of infected rat serum the antibody-dependent cell-mediated cytotoxicity of peritoneal macrophages on metacercariae was significantly increased and showed a peak at 6-hour incubation. But the cytotoxic effect was markedly reduced after inactivation of complement and heat-labile IgE antibody by the heating of infected serum at 56℃ for 30 minutes.

3. The highest cytotoxic effect (100%) of concomitant incubation with IgG and complement showed 24 hours after incubation, although cell adherence was relatively low at 6-hour incubation and 0% at 24-hour incubation.

4. Coordinative functions of complement with serum and IgG were effective in cell adherence and in cytotoxicity, but it is not clear the independent role of complement on the macrophage-mediated cytotoxicity in this study.

With these results it is assumed that P. westermani infection can induce the non-specific activation of peritoneal macrophages, and serum antibodies including IgE antibody might enhance the cytotoxicity by macrophages.

Figures


Fig. 1
P. westermani metacercariae incubated with rat peritoneal macrophages in RPMI/FCS. After 6-hour incubation in various conditions cell adherence was classified as negative response (a), moderate (b) or extensive attachment (c).


Fig. 2
The peritoneal macrophage adherence on P. westermani metacercaria after incubation with various conditions.

(▵-▵: cells from normal rat, ▪-▪: cells from infected rat, ✕-✕: cells from normal rat + normal rat serum, •-•: cells from infected rat + normal rat serum).



Fig. 3
The effects of fresh and inactivated sera from P. westermani infected rat after incubation with normal peritoneal macrophages.

(•-•: normal rat serum, ▪-▪: infected rat serum, ◦-◦: heat-inactivated infected serum at 56℃ for 30 minutes).


Tables


Table 1
The percent of metacercariae showing peritoneal macrophage adherence after being incubated with cells, sera or IgG antibody from normal rats or rats infected with P. westermani


Table 2
The number of non-motile metacercariae after incubation with anti-serum or IgG antibody and peritoneal macrophages from normal rat


Table 3
Motility changes of survived metacercariae after incubation with antiserum or IgG antibody and peritoneal macrophages from normal rat


Table 4
The percent of metacercariae showing peritoneal macrophage adherence after being incubated with cells and sera from paragonimiasis patient

References
1. Butterworth AE, Sturrock RF, Houba V, Mahmoud AA, Sher A, Rees PH. Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature 1975;256(5520):727–729.
  
2. Capron A, Dessaint JP, Capron M, Bazin H. Specific IgE antibodies in immune adherence of normal macrophages to Schistosoma mansoni schistosomules. Nature 1975;253(5491):474–475.
  
3. Capron A, Dessaint JP, Haque A, Auriault C, Joseph M. Macrophages as effector cells in helminth infections. Trans R Soc Trop Med Hyg 1983;77(5):631–635.
  
4. Chung PR, Asch HL, Bruce JI. Antibody-dependent murine macrophage-mediated damage to Schistosoma mansoni schistosomula in vitro. Cell Immunol 1982;74(2):243–259.
  
5. Davis CE, et al. J Immunol 1988;41:627–635.
6. Dean DA, Wistar R, Chen P. Immune response of guinea pigs to Schistosoma mansoni. I. In vitro effects of antibody and neutrophils, eosinophils and macrophages on schistosomula. Am J Trop Med Hyg 1975;24(1):74–82.
 
7. Dessaint JP, Torpier G, Capron M, Bazin H, Capron A. Cytophilic binding of IgE to the macrophage. I. Binding characteristics of IgE on the surface of macrophages in the rat. Cell Immunol 1979;46(1):12–23.
  
8. Haque A, Joseph M, Ouaissi MA, Capron M, Capron A. IgE antibody-mediated cytotoxicity of rat macrophages against microfilaria of Dipetalonema citeae in vitro. Clin Exp Immunol 1980;40(3):487–495.
 
9. Haque A, Ouaissi A, Joseph M, Capron M, Capron A. IgE antibody in eosinophil- and macrophage-mediated in vitro killing of Dipetalonema viteae microfilariae. J Immunol 1981;127(2):716–725.
 
10. Haque A, Ouaissi A, Santoro F, des Moutis I, Capron A. Complement-mediated leukocyte adherence to infective larvae of Dipetalonema viteae (Filarioidea): requirement for eosinophils or eosinophil products in effecting macrophage adherence. J Immunol 1982;129(5):2219–2225.
 
11. James SL, Sher A, Lazdins JK, Meltzer MS. Macrophages as effector cells of protective immunity in murine schistosomiasis. II. Killing of newly transformed schistosomula in vitro by macrophages activated as a consequence of Schistosoma mansoni infection. J Immunol 1982;128(4):1535–1540.
 
12. Jarrett EE. Helminth infection and the biosynthesis of IgE antibodies. Trans R Soc Trop Med Hyg 1972;66(4):546.
 
13. Jarrett E, Bazin H. Elevation of total serum IgE in rats following helminth parasite infection. Nature 1974;251(5476):613–614.
  
14. Joseph M, Capron A. IgE receptors on macrophages: biological significance. Agents Actions 1985;16(1-2):27–29.
  
15. Kojima S, Yokogawa M, Tada T. Raised levels of serum IgE in human helminthiases. Am J Trop Med Hyg 1972;21(6):913–918.
 
16. Kubelka C, et al. Immunobiol 1982;162:382.
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
 
18. Mahmoud AA, Peters PA, Civil RH, Remington JS. In vitro killing of schistosomula of Schistosoma mansoni by BCG and C. parvum-activated macrophages. J Immunol 1979;122(5):1655–1657.
 
19. Min DY, Soh CT. Evaluation Of Specific Ige Antibody In Clonorchis Sinensis Infection. Korean J Parasitol 1983;21(1):27–31.
 
20. Nacy CA, Fortier AH, Meltzer MS, Buchmeier NA, Schreiber RD. Macrophage activation to kill Leishmania major: activation of macrophages for intracellular destruction of amastigotes can be induced by both recombinant interferon-gamma and non-interferon lymphokines. J Immunol 1985;135(5):3505–3511.
 
21. Oster CN, Nacy CA. Macrophage activation to kill Leishmania tropica: kinetics of macrophage response to lymphokines that induce antimicrobial activities against amastigotes. J Immunol 1984;132(3):1494–1500.
 
22. Oxenham SL, Mackenzie CD, Denham DA. Increased activity of macrophages from mice infected with Brugia pahangi: in vitro adherence to microfilariae. Parasite Immunol 1984;6(2):141–156.
  
23. Perez HA, Smithers SR. Schistosoma mansoni in the rat: the adherence of macrophages to schistosomula in vitro after sensitization with immune serum. Int J Parasitol 1977;7(4):315–320.
  
24. Pestel J, Joseph M, Dessaint JP, Capron A. Macrophage triggering by aggregated immunoglobulins. I. Delayed effect of IgG aggregates or immune complexes. J Immunol 1981;126(5):1887–1891.
 
25. Yokogawa M, Kojima S, Araki K, Tomioka H, Yoshida S. Immunoglobulin E: raised levels in sera and pleural exudates of patients with paragonimiasis. Am J Trop Med Hyg 1976;25(4):581–586.
 
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