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Clinical and histopathological findings in mice heavily infected with Fibricola seoulensis
Sun Huh,Jong Yil Chai,Sung Tae Hong and Soon Hyung Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-460, Korea.
Abstract
To observe the clinical course and intestinal histopathology in heavy infection of Fibricola seoulensis, an experimental study was performed in mice. Clinical, hematological and histopathological observation was done on 1, 3, 7 and 12 days after experimental infection with l,000 metacercariae. On the 11th day after infection, the mice began to die and all of the infected mice were dead by the 16th day. The infected mice showed gradual weight loss. Occult blood was detected after the 10th day. Diarrhea occurred after the 9th day and was recognized in all of the infected mice on the 12th day. Hemoglobin and mean corpuscular hemoglobin decreased significantly after the 12th day, and mean corpuscular hemoglobin concentration decreased in all infected mice. The histopathological changes were more marked in the duodenum than in the jejunum or ileum. Major changes were villous atrophy and crypt hyperplasia, with decreased villus/crypt ratio, inflammatory cell infiltration and stromal edema. The present results suggest that the cause of death of mice heavily infected with F. seoulensis should be malnutrition and severe fluid loss due to malabsorption, together with intestinal bleeding.
Figures
Fig. 1 Survival rate of mice fed 1,000 metacercariae of F. seoulensis, in comparison with control group.
Fig. 2 Weight change of mice fed 1,000 metacercariae of F. seoulensis, in comparison with control group. (工 ; standard deviation)
Figs. 3-11 Fig. 3. The gross feature of an intestinal segment of a mouse dead on day 16 after infection, showing gross luminal bleeding and worms (arrows).
Fig. 4. Mouse duodenum of control group, showing normal and long, finger-like villi. H-E stain, ×100.
Fig. 5. Duodenal section of a 1-day infected mouse showing a young fluke in the intervillous space, H-E stain, ×100.
Fig. 6. Duodenal section of a 3-day infected mouse showing a worm entrapping the tip of a villus. Destruction of epithelial cells adjacent to the worm, and compression of the villus is conspicious. H-E stain, ×100.
Fig. 7. Duodenal section of a 7-day infected mouse showing villous atrophy, crypt hyperplasia, cellular infiltration and decreased V/C ratio (about 1:1). H-E stain, ×40.
Fig. 8. Duodenal section of a 7-day infected mouse showing a mature worm entrapping the villus, and showing destroyed villi. H-E stain, ×100.
Fig. 9. Jejunal section of a 7-day infected mouse showing stromal edema and decreased V/C ratio. H-E stain, ×40.
Fig. 10. Duodenal section of a 12-day infected mouse showing marked villous atrophy, crypt hyperplasia and reversed V/C ratio (1:2). H-E stain, ×40.
Fig. 11. Ileal section of a 12-day infected mouse showing severe stromal edema and flatting of villi and markedly decreased V/C ratio (2:1 to 1:1). H-E stain, ×100.
Tables
Table 1 Occult blood positive rate in mice fed 1,000 metacercariae of F. seoulensis by infection day
Table 2 Appearance rate of diarrhea in mice fed 1,000 metacercariae of F. seoulensis by infection day
Table 3 Number of recovered worms from each mouse according to duration of survival days
Table 4 Hematological values of mice fed 1,000 metacercariae of F. seoulensis by day
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