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Korean J Parasitol > Volume 25(2):1987 > Article

Original Article
Korean J Parasitol. 1987 Dec;25(2):188-194. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1987.25.2.188
Copyright © 1987 by The Korean Society for Parasitology
Serological cross-reactivity between Sarcocystis and Toxoplasma in pigs
Moo Hong Moon
Department of Veterinary Medicine, College of Agriculture, Kyungpook National University, Daegu 635, Korea.
Abstract

The development of antibody titers and cross reation between Sarcocystis and Toxoplasma were investigated by means of IFA test and ELISA in pigs experimentally infected with 1.5 × 10(6) S. suicanis sporocysts and 10,000 T. gondii oocysts, respectively. The intact and soluble Sarcocystis antigens were prepared from the bradyzoites harvested by peptic digestion of infected pork. The intact and soluble Toxoplasma, antigens were prepared from the tachyzoites in mouse peritoneal cavity. IgG antibodies in pigs infeded with Sarcocystis and Toxoplasma, respectively were detected first at 2 weeks post infection on both IFA test and ELISA. The antibody titer to Toxoplasma reached its maximum at 6 weeks post infection and decreased thereafter. The antibody titer to Sarcocystis reached its maximum terminally. The cross-reaction titer in pigs infected with Toxoplasma against Sarcocystis antigen was up to 1:16 in IFA test and up to 1:32 in ELISA. The titer in control group was below 1:4 in both reactions.

Figures


Fig. 1
Dynamics of IgG antibody of pigs infected with Sarcocystis (…)and Toxoplasma (-) to homologous antigens, and Toxoplasma (-•-) to Sarcocystis antigen by IFA test.


Fig. 2
Dynamics of IgG antibody of pigs infected with Sarcocystis (…)and Toxoplasma (-) to homologous antigens, and Toxoplasma (-•-) to Sarcocystis antigen by ELISA.

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