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Histopathological changes of the liver after praziquantel treatment in Clonorchis sinensis infected rabbits
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Korean J Parasito > Volume 25(2):1987 > Article

Original Article
Korean J Parasitol. 1987 Dec;25(2):110-122. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1987.25.2.110
Copyright © 1987 by The Korean Society for Parasitology
Histopathological changes of the liver after praziquantel treatment in Clonorchis sinensis infected rabbits
Soon Hyung Lee,Sung Tae Hong,Chong Soon Kim,Woon Mok Sohn,Jong Yil Chai and Yoon Seong Lee*
Department of Parasitology, College of Medicine, Seoul National University, Seoul 110, Korea.
*Department of Forensic Medicine and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110, Korea.
Abstract

The rabbits were infected with Clnorchis sinensis and were treated with praziquantel at the dose of 50 mg/kg × 2 × 2 days afer 1, 2, 4, 8 weeks and 7 months from the infection. Their livers were observed histopathologically 1, 4 and 12 weeks after treatment. The findings are summarized as below: The changes of the liver in control rabbits were relatively mild until 2 weeks after infection. However, widening and thickening of bile ducts, proliferation of biliary epithelium and periductal fibrosis were moderate after 4 weeks from infection and those changes were severe after 8 weeks and 7months. Goblet cell metaplasia was found after 8 weeks from infection. The mild changes of 2-week infection group were completely recovered by 4 weeks after the treatment. In the groups of 4 or more weeks after infection, the changes of bile ducts became milder in the degree after the treatment, but were still found 12 weeks after the treatment. As the infection duration was passed, more severe changes were observed after the treatment. In this context, it is concluded that the liver changes of acute clonorchiasis in the early two weeks are reversible by treatment while chronic biliary epithelial changes are irreversible. Therefore, early treatment should be recommended as possible to minimize the remaining histopathological changes of liver in clonorchiasis.

Figures


Figs. 1-4
Fig. 1. Infected control of 1 week infection showing a juvenile C. sinensis in a small bile duct, and periductal edema, hematoxylin-eosin (HE) stained, ×140.

Fig. 2. Two large ducts after 4 weeks from treatment of 1 week infection with slight periductal inflammation, HE stained, ×100.

Fig. 3. Infected control of 2 week infection showing a worm in a dilated bile duct. Papillary proliferation of the duct epithelia and periductal edema and inflammation were observed, HE stained, ×40.

Fig. 4. High power view of a bile duct 12 weeks after treatment of 2 week infection with slight periductal inflammation, HE stained, ×200.



Figs. 5-8
Fig. 5. Infected control of 4 week infection, 2 sections of mature worms were found in a dilated bile duct. Biliary epithelial layer was compressed, flattened or desquamated near the worms, HE stained, ×48.

Fig. 6. Small bile ducts 1 week after treatment of 4 week infection with slight epithelial proliferation and periductal fibrosis, HE stained, ×72.

Fig. 7. A large bile duct 12 weeks after treatment of 4 week infection showing slight epithelial proliferation and periductal inflammation, HE stained, ×48.

Fig. 8. Three sections of C. sinensis in a severely dilated bile duct after 8 week from infection. Epithelial proliferation and compression, desquamation and periductal inflammation were observed, HE stained, ×40.



Figs.9-12
Fig. 9. A large bile duct a without worm of a 8 week infected control rabbit, with papillary or glandular proliferation of epithelium, HE stained, ×40.

Fig. 10. A large duct one week after treatment of 8 week infection with irregular epithelial proliferation and periductal fibrosis, HE stained, ×40.

Fig. 11. A large duct 4 weeks after treatment of 8 week infection with irregular epithelial proliferation and periductal fibrosis, HE stained, ×56.

Fig. 12. A large duct 12 weeks after treatment of 8 week infection with slight epithelial proliferation and periductal fibrosis, HE stained, ×56.



Figs. 13-16
Fig. 13. A sectioned worm of mature C. sinensis in a dilated bile duct was found 7 months after infection. Epithelial layer of the duct was proliferated markedly and desquamated partly, HE stained, ×40.

Fig. 14. A large duct with papillary and adenomatous hyperplasia of epithelium and periductal fibrosis and inflammation, of 7 month infection, HE stained, ×40.

Fig. 15. High power of a bile duct 7 months infected control showing goblet cells in biliary epithelium, HE stained, ×200.

Fig. 16. A large bile duct 4 weeks after treatment of 7 month infection showing papillary and adenomatous hyperplasia and periductal fibrosis, HE stained, ×56.


Tables


Table 1
Number of rabbits infected by C. sinensis* and treated with praziquantel**


Table 2
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 1 week duration of infection


Table 3
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 2 week duration of infection


Table 4
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 4 week duration of infection


Table 5
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 8 week duration of infection


Table 6
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 7 month duration of infection

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