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Korean J Parasitol > Volume 21(1):1983 > Article

Original Article
Korean J Parasitol. 1983 Jun;21(1):1-5. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1983.21.1.1
Copyright © 1983 by The Korean Society for Parasitology
Thin layer immunoassay (TIA) for circulating antibody detection in clonorchiasis
Kyung-Il Im
Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.

Thin layer immunoassay was carried out to demonstrate antibodies against Clonorchis sinensis in sera from clonorchiasis patients. Saline extract of adult worm was used as antigen. TIA technique was performed as described earlier by Elwing et al. (1976), but agarose was used instead of agar. The antibody titres of sera in 60 clonorchiasis cases were higher than that of 10 healthy and 10 amoebiasis cases, but not different comparing with that of 10 paragonimiasis cases. Antibody titres in clonorchiasis gave no differences according to the age, sex, EPG in feces, eosinophilia degree of blood, level of alkaline phosphatase and transaminase (SGOT, SGPT) in sera.

It is suggested that, after evaluation, the TIA might supplement or be used as an alternative to other immunodiagnostic tests already in use for the diagnosis of clonorchiasis.


Fig. 1
Antibody titres distribution by TIA in 60 clonorchiasis, 10 healthy, 10 paragonimiasis and 10 amoebiasis cases.

Fig. 2
Antibody titres distribution by TIA in clonorchiasis cases by eggs per gram of feces (EPG).

Fig. 3
Antibody titres in clonorchiasis by percentage of eosinophilic leukocyte.

Fig. 4
Antibody titres by alkaline phosphatase activity in serum of clonorchiasis cases.

Fig. 5
Antibody tetres by TIA with reference to S.G.O.T. in clonorchiasis cases.

Fig. 6
Antibody titres by TIA with reference to S.G.P.T. in clonorchiasis cases.


Table 1
Comparison of antibody titres examined by Thin Layer Immunoassay (TIA) in clonorchiasis and other parasitic diseases

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